| Literature DB >> 18768310 |
M Yakup Arica1, Begum Altintas, Gülay Bayramoğlu.
Abstract
Non-porous poly(glycidyl methacrylate/ethyleneglycol dimetacrylate) (poly(GMA/EGDMA)) beads were prepared by suspension polymerization. The enzyme (i.e. laccase) was covalently immobilized onto plain and spacer-arm attached poly(GMA/EGDMA) beads. The amount of immobilized enzyme on the plain and spacer-arm attached beads was determined as 5.6 and 4.9 mg/g, respectively. The maximum activity (V(max)) and Michaelis constant (K(m)) of laccase immobilized on the spacer-arm attached beads, were found to be 77.6 U/min and 0.47 mM, respectively. Finally, the immobilized laccase was operated in a batch system, and textile dye Reactive Red 120 was successfully decolorized in the enzyme reactor.Entities:
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Year: 2008 PMID: 18768310 DOI: 10.1016/j.biortech.2008.07.038
Source DB: PubMed Journal: Bioresour Technol ISSN: 0960-8524 Impact factor: 9.642