AIMS: Studies have shown that erythrocyte membranes are present within necrotic cores in atherosclerotic plaques, and that circulating erythrocytes in patients with acute coronary syndrome (ACS) have increased total cholesterol content (CEM). Interleukin-8 (IL-8) binds to erythrocytes and during intraplaque haemorrhage it is released into the plaque and thus may contribute to inflammatory cascade and atherosclerotic plaque instability. The present study was undertaken to test the hypothesis that erythrocyte membrane IL-8 is elevated in patients with ACS compared with those with chronic stable angina (CSA). METHODS AND RESULTS: Consecutive patients who presented with CSA (n = 120, 92 men, 62 +/- 9 years), ACS (n = 118, 90 men, 62 +/- 10 years) or with chest pain who had normal coronary arteries (n = 36, 26 men, 60 +/- 7 years), were studied prospectively. IL-8 concentrations in erythrocyte membranes (rIL-8) and in plasma (pIL-8), C-reactive protein (CRP) and CEM were measured. rIL-8 levels [mean +/- 1 SD (standard deviation)] were higher in ACS (102.9 +/- 70.1 pg/mL) compared with CSA (44.7 +/- 22.8 pg/mL) (P < 0.001). No difference in pIL-8 levels between the two coronary artery disease groups was observed (P = 0.280). Serum CRP levels were correlated with rIL-8 levels (r = 0.294, P < 0.001); no association was found between CRP and pIL-8 levels (r = 0.025, P = 0.706). Further, rIL-8 had an independent association with ACS, when CRP and CEM were taken into consideration. CONCLUSION: This study shows for the first time that rIL-8 content was significantly higher in ACS, compared with CSA. These findings endorse results from our previous studies suggesting that erythrocytes may play an important role in the development of unstable atherosclerotic plaque.
AIMS: Studies have shown that erythrocyte membranes are present within necrotic cores in atherosclerotic plaques, and that circulating erythrocytes in patients with acute coronary syndrome (ACS) have increased total cholesterol content (CEM). Interleukin-8 (IL-8) binds to erythrocytes and during intraplaque haemorrhage it is released into the plaque and thus may contribute to inflammatory cascade and atherosclerotic plaque instability. The present study was undertaken to test the hypothesis that erythrocyte membrane IL-8 is elevated in patients with ACS compared with those with chronic stable angina (CSA). METHODS AND RESULTS: Consecutive patients who presented with CSA (n = 120, 92 men, 62 +/- 9 years), ACS (n = 118, 90 men, 62 +/- 10 years) or with chest pain who had normal coronary arteries (n = 36, 26 men, 60 +/- 7 years), were studied prospectively. IL-8 concentrations in erythrocyte membranes (rIL-8) and in plasma (pIL-8), C-reactive protein (CRP) and CEM were measured. rIL-8 levels [mean +/- 1 SD (standard deviation)] were higher in ACS (102.9 +/- 70.1 pg/mL) compared with CSA (44.7 +/- 22.8 pg/mL) (P < 0.001). No difference in pIL-8 levels between the two coronary artery disease groups was observed (P = 0.280). Serum CRP levels were correlated with rIL-8 levels (r = 0.294, P < 0.001); no association was found between CRP and pIL-8 levels (r = 0.025, P = 0.706). Further, rIL-8 had an independent association with ACS, when CRP and CEM were taken into consideration. CONCLUSION: This study shows for the first time that rIL-8 content was significantly higher in ACS, compared with CSA. These findings endorse results from our previous studies suggesting that erythrocytes may play an important role in the development of unstable atherosclerotic plaque.
Authors: Imo E Hoefer; Jan-Willem Sels; J Wouter Jukema; Sandrin Bergheanu; Erik Biessen; Elizabeth McClellan; Mat Daemen; Pieter Doevendans; Philip de Groot; Marieke Hillaert; Sebastiaan Horsman; Mustafa Ilhan; Johan Kuiper; Nico Pijls; Ken Redekop; Peter van der Spek; Andrew Stubbs; Eric van de Veer; Johannes Waltenberger; Anton-Jan van Zonneveld; Gerard Pasterkamp Journal: Clin Res Cardiol Date: 2013-08-23 Impact factor: 5.460
Authors: Dawn M Fernandez; Adeeb H Rahman; Nicolas F Fernandez; Aleksey Chudnovskiy; El-Ad David Amir; Letizia Amadori; Nayaab S Khan; Christine K Wong; Roza Shamailova; Christopher A Hill; Zichen Wang; Romain Remark; Jennifer R Li; Christian Pina; Christopher Faries; Ahmed J Awad; Noah Moss; Johan L M Bjorkegren; Seunghee Kim-Schulze; Sacha Gnjatic; Avi Ma'ayan; J Mocco; Peter Faries; Miriam Merad; Chiara Giannarelli Journal: Nat Med Date: 2019-10-07 Impact factor: 53.440
Authors: Elsa Wilma Böhm; Maria Pavlaki; Georgios Chalikias; Dimitrios Mikroulis; George S Georgiadis; Dimitrios N Tziakas; Stavros Konstantinides; Katrin Schäfer Journal: TH Open Date: 2021-04-14
Authors: Jan-Willem E M Sels; Ellen H A M Elsenberg; Imo E Hoefer; Anton Jan van Zonneveld; Johan Kuiper; J Wouter Jukema; Nico H J Pijls; Gerard Pasterkamp Journal: PLoS One Date: 2012-10-16 Impact factor: 3.240