[The mitochondrial permeability transition pore opening under oxidative stress in ischemia/reperfusion in the tissue of the lower extremities].

The study was conducted within a group of 17 patients suffering from thrombosis in the femoral artery to identify a mitochondrial factor release into the blood at ischemia/reperfusion in the lower extremities. To test the mitochondrial factor as a possible marker of the mitochondrial permeability transition pore opening under oxidative stress we determined the rates ofoxidative stress (malonic dyaldehyde, MDA), oxidative protection (catalase) and mitochondrial factor release under ischemia/ reperfusion induced by surgical operation. It has been shown that the parameters of pro- and antioxidant systems changed in a way characteristic of oxidative stress: content of MDA in the blood at reperfusion increased, but activity of catalase decreased. We were the first to record the mitochondrial factor release into the blood of the femoral artery on the wave length of 245 nm using spectrophotometry. The data obtained indicate that mitochondrial factor might serve as an effective way of diagnostics of oxidative stress in injured tissues at ischemia/ reperfusion in the lower extremities.