BACKGROUND: Bone marrow mesenchymal stem cell (MSC) transplantation is a promising strategy in the treatment of myocardial infarction (MI). However, the time for transplanting cells remains controversial. The aim of this study was to find an optimal time point for cell transplantation. METHODS: MSCs were isolated and cultured from Sprague-Dawley (SD) rats. MI model was set up in SD rats by permanent ligation of left anterior descending coronary artery. MSCs were directly injected into the infarct border zone at 1 h, 1 week and 2 weeks after MI, respectively. Sham-operated and MI control groups received equal volume of phosphate buffered saline (PBS). At 4 weeks after MI, cardiac function was assessed by echocardiography; vessel density was analyzed on hematoxylin-eosin stained slides by light microscopy; the apoptosis of cardiomyocytes was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay; the expressions of proteins were analyzed by Western blot. RESULTS: MSC transplantation improved cardiac function, reduced the apoptosis of cardiomyocytes and increased vessel density. These benefits were more obvious in 1-week group than in 1-h and 2-week groups. There are more obvious increases in the ratio of bcl-2/bax and the expression of vascular endothelial growth factor (VEGF) and more obvious decreases in the expression of cleaved-caspase-3 in 1-week group than those in other two groups. CONCLUSION: MSC transplantation was beneficial for the recovery of cardiac function. MSC transplantation at 1 week post-MI exerted the best effects on increases of cardiac function, anti-apoptosis and angiogenesis.
BACKGROUND: Bone marrow mesenchymal stem cell (MSC) transplantation is a promising strategy in the treatment of myocardial infarction (MI). However, the time for transplanting cells remains controversial. The aim of this study was to find an optimal time point for cell transplantation. METHODS: MSCs were isolated and cultured from Sprague-Dawley (SD) rats. MI model was set up in SD rats by permanent ligation of left anterior descending coronary artery. MSCs were directly injected into the infarct border zone at 1 h, 1 week and 2 weeks after MI, respectively. Sham-operated and MI control groups received equal volume of phosphate buffered saline (PBS). At 4 weeks after MI, cardiac function was assessed by echocardiography; vessel density was analyzed on hematoxylin-eosin stained slides by light microscopy; the apoptosis of cardiomyocytes was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay; the expressions of proteins were analyzed by Western blot. RESULTS: MSC transplantation improved cardiac function, reduced the apoptosis of cardiomyocytes and increased vessel density. These benefits were more obvious in 1-week group than in 1-h and 2-week groups. There are more obvious increases in the ratio of bcl-2/bax and the expression of vascular endothelial growth factor (VEGF) and more obvious decreases in the expression of cleaved-caspase-3 in 1-week group than those in other two groups. CONCLUSION: MSC transplantation was beneficial for the recovery of cardiac function. MSC transplantation at 1 week post-MI exerted the best effects on increases of cardiac function, anti-apoptosis and angiogenesis.
Authors: Birgit Assmus; Volker Schächinger; Claudius Teupe; Martina Britten; Ralf Lehmann; Natascha Döbert; Frank Grünwald; Alexandra Aicher; Carmen Urbich; Hans Martin; Dieter Hoelzer; Stefanie Dimmeler; Andreas M Zeiher Journal: Circulation Date: 2002-12-10 Impact factor: 29.690
Authors: Bodo E Strauer; Michael Brehm; Tobias Zeus; Matthias Köstering; Anna Hernandez; Rüdiger V Sorg; Gesine Kögler; Peter Wernet Journal: Circulation Date: 2002-10-08 Impact factor: 29.690
Authors: Wilson A Silva; Dimas T Covas; Rodrigo A Panepucci; Rodrigo Proto-Siqueira; Jorge L C Siufi; Dalila L Zanette; Anemari R D Santos; Marco A Zago Journal: Stem Cells Date: 2003 Impact factor: 6.277
Authors: Christof Stamm; Bernd Westphal; Hans-Dieter Kleine; Michael Petzsch; Christian Kittner; Heiko Klinge; Carl Schümichen; Christoph A Nienaber; Mathias Freund; Gustav Steinhoff Journal: Lancet Date: 2003-01-04 Impact factor: 79.321
Authors: Andrey A Karpov; Yulia K Uspenskaya; Sarkis M Minasian; Maxim V Puzanov; Renata I Dmitrieva; Anna A Bilibina; Sergey V Anisimov; Michael M Galagudza Journal: Int J Exp Pathol Date: 2013-04-08 Impact factor: 1.925