Literature DB >> 18763306

Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector.

Jin-zhong Xu1, Jie Shen, Yi-yu Cheng, Hai-bin Qu.   

Abstract

A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008-0.160 microg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.

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Year:  2008        PMID： 18763306      PMCID： PMC2528888          DOI： 10.1631/jzus.B0820095

Source DB:  PubMed          Journal:  J Zhejiang Univ Sci B        ISSN： 1673-1581            Impact factor:   3.066


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