Yu-bin Ji1, Shi-yong Gao. 1. Postdoctoral Research Station of the Institute of Materia Medica, Center for Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China.
Abstract
OBJECTIVE: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. METHODS: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. RESULTS: NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells. CONCLUSION: NAT can inhibit apoptosis in HepG2 cells.
OBJECTIVE: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. METHODS: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. RESULTS: NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells. CONCLUSION: NAT can inhibit apoptosis in HepG2 cells.
Authors: Peter V Bozhkov; Maria F Suarez; Lada H Filonova; Geoffrey Daniel; Andrey A Zamyatnin; Salvador Rodriguez-Nieto; Boris Zhivotovsky; Andrei Smertenko Journal: Proc Natl Acad Sci U S A Date: 2005-09-23 Impact factor: 11.205