Carolyn Cray1, David Villar. 1. Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, Miami, FL 33101, USA. c.cray@miami.edu
Abstract
BACKGROUND: A major challenge in the serologic diagnosis of infectious diseases in exotic birds is the limited availability of species-specific antibodies. OBJECTIVES: The purpose of the current study was to determine if there is cross reactivity between commercially available anti-chicken IgY antibodies and immunoglobulins of several avian species, with particular emphasis on psittacines. METHODS: To quantitate the reactivity with anti-chicken IgY, Western blot analysis was performed using plasma samples from many different avian species. Results were compared with gamma globulin fraction quantitation obtained by protein electrophoresis. RESULTS: By Western blot, 2 protein bands corresponding to the heavy and light chains of chicken IgY were identified in species from 21 avian orders using 1 of 2 rabbit anti-chicken IgY antibodies. Densitometric analysis showed that the amount of immunoglobulin estimated from Western blots correlated strongly with data from protein electrophoresis assays. CONCLUSIONS: The results demonstrate that some commercially available anti-chicken IgY antibodies exhibit good cross-reactivity with most avian species.
BACKGROUND: A major challenge in the serologic diagnosis of infectious diseases in exotic birds is the limited availability of species-specific antibodies. OBJECTIVES: The purpose of the current study was to determine if there is cross reactivity between commercially available anti-chicken IgY antibodies and immunoglobulins of several avian species, with particular emphasis on psittacines. METHODS: To quantitate the reactivity with anti-chicken IgY, Western blot analysis was performed using plasma samples from many different avian species. Results were compared with gamma globulin fraction quantitation obtained by protein electrophoresis. RESULTS: By Western blot, 2 protein bands corresponding to the heavy and light chains of chicken IgY were identified in species from 21 avian orders using 1 of 2 rabbit anti-chicken IgY antibodies. Densitometric analysis showed that the amount of immunoglobulin estimated from Western blots correlated strongly with data from protein electrophoresis assays. CONCLUSIONS: The results demonstrate that some commercially available anti-chicken IgY antibodies exhibit good cross-reactivity with most avian species.
Authors: Carol A Fassbinder-Orth; Travis E Wilcoxen; Tiffany Tran; Raoul K Boughton; Jeanne M Fair; Erik K Hofmeister; Jennifer L Grindstaff; Jen C Owen Journal: Methods Ecol Evol Date: 2016-05-28 Impact factor: 7.781