OBJECTIVE: To evaluate in vivo the evolution of osteoarthritis (OA) lesions temporally in a rabbit model of OA with clinically available imaging modalities: computed radiography (CR), helical single-slice computed tomography (CT), and 1.5 tesla (T) magnetic resonance imaging (MRI). METHODS: Imaging was performed on knees of anesthetized rabbits [10 anterior cruciate ligament transection (ACLT) and contralateral sham joints and six control rabbits] at baseline and at intervals up to 12 weeks post-surgery. Osteophytosis, subchondral bone sclerosis, bone marrow lesions (BMLs), femoropatellar effusion and articular cartilage were assessed. RESULTS: CT had the highest sensitivity (90%) and specificity (91%) to detect osteophytes. A significant increase in total joint osteophyte score occurred at all time-points post-operatively in the ACLT group alone. BMLs were identified and occurred most commonly in the lateral femoral condyle of the ACLT joints and were not identified in the tibia. A significant increase in joint effusion was present in the ACLT joints until 8 weeks after surgery. Bone sclerosis or cartilage defects were not reliably assessed with the selected imaging modalities. CONCLUSION: Combined, clinically available CT and 1.5 T MRI allowed the assessment of most of the characteristic lesions of OA and at early time-points in the development of the disease. However, the selected 1.5 T MRI sequences and acquisition times did not permit the detection of cartilage lesions in this rabbit OA model.
OBJECTIVE: To evaluate in vivo the evolution of osteoarthritis (OA) lesions temporally in a rabbit model of OA with clinically available imaging modalities: computed radiography (CR), helical single-slice computed tomography (CT), and 1.5 tesla (T) magnetic resonance imaging (MRI). METHODS: Imaging was performed on knees of anesthetized rabbits [10 anterior cruciate ligament transection (ACLT) and contralateral sham joints and six control rabbits] at baseline and at intervals up to 12 weeks post-surgery. Osteophytosis, subchondral bone sclerosis, bone marrow lesions (BMLs), femoropatellar effusion and articular cartilage were assessed. RESULTS: CT had the highest sensitivity (90%) and specificity (91%) to detect osteophytes. A significant increase in total joint osteophyte score occurred at all time-points post-operatively in the ACLT group alone. BMLs were identified and occurred most commonly in the lateral femoral condyle of the ACLT joints and were not identified in the tibia. A significant increase in joint effusion was present in the ACLT joints until 8 weeks after surgery. Bone sclerosis or cartilage defects were not reliably assessed with the selected imaging modalities. CONCLUSION: Combined, clinically available CT and 1.5 T MRI allowed the assessment of most of the characteristic lesions of OA and at early time-points in the development of the disease. However, the selected 1.5 T MRI sequences and acquisition times did not permit the detection of cartilage lesions in this rabbit OA model.
Authors: James R Pinney; Carmen Taylor; Ryan Doan; Andrew J Burghardt; Xiaojuan Li; Hubert T Kim; C Benjamin Ma; Sharmila Majumdar Journal: Magn Reson Imaging Date: 2011-11-08 Impact factor: 2.546
Authors: A J Grodzinsky; R M Porter; A G Bajpayee; R E De la Vega; M Scheu; N H Varady; I A Yannatos; L A Brown; Y Krishnan; T J Fitzsimons; P Bhattacharya; E H Frank Journal: Eur Cell Mater Date: 2017-12-05 Impact factor: 3.942
Authors: Hannah M Pauly; Blair E Larson; Garrett A Coatney; Keith D Button; Charlie E DeCamp; Ryan S Fajardo; Roger C Haut; Tammy L Haut Donahue Journal: J Orthop Res Date: 2015-07-17 Impact factor: 3.494
Authors: Andrew Bowen; David Shamritsky; Josue Santana; Ian Porter; Erica Feldman; Sarah L Pownder; Matthew F Koff; Kei Hayashi; Christopher J Hernandez Journal: JBMR Plus Date: 2022-02-15