OBJECTIVE: To investigate the potentially useful of platelet-rich plasma (PRP) on mRNA expression of angiogenesis. STUDY DESIGN: Adjunct assay and reverse-transcription polymerase chain reaction (RT-PCR) analysis of type I collagen, vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) in rat bone marrow stromal cells differentiation in 14 days' culture. RESULTS: The PRP significantly elevated alkaline phosphatase activity after day 5 (P < .05), and DNA and protein content increased at culture days 1, 3, and 5 (P < .01) with PRP compared with control. The RT-PCR demonstrated that type I collagen was expressed in all substrates and remained high with PRP during 14 days of culture, and that mRNA expression of VEGF and PDGF were higher over time. CONCLUSIONS: This study indicates a potential contribution of PRP as possibly starting the process of angiogenesis, recruiting the endothelial cells which line blood vessels, and beginning the initiation of bone regeneration.
OBJECTIVE: To investigate the potentially useful of platelet-rich plasma (PRP) on mRNA expression of angiogenesis. STUDY DESIGN: Adjunct assay and reverse-transcription polymerase chain reaction (RT-PCR) analysis of type I collagen, vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) in rat bone marrow stromal cells differentiation in 14 days' culture. RESULTS: The PRP significantly elevated alkaline phosphatase activity after day 5 (P < .05), and DNA and protein content increased at culture days 1, 3, and 5 (P < .01) with PRP compared with control. The RT-PCR demonstrated that type I collagen was expressed in all substrates and remained high with PRP during 14 days of culture, and that mRNA expression of VEGF and PDGF were higher over time. CONCLUSIONS: This study indicates a potential contribution of PRP as possibly starting the process of angiogenesis, recruiting the endothelial cells which line blood vessels, and beginning the initiation of bone regeneration.
Authors: Matthew B Murphy; Daniel Blashki; Rachel M Buchanan; Dongmei Fan; Enrica De Rosa; Ramille N Shah; Samuel I Stupp; Bradley K Weiner; Paul J Simmons; Mauro Ferrari; Ennio Tasciotti Journal: J Funct Biomater Date: 2011-06-21
Authors: Richard J Griffeth; Daniel García-Párraga; Maravillas Mellado-López; Jose Luis Crespo-Picazo; Mario Soriano-Navarro; Alicia Martinez-Romero; Victoria Moreno-Manzano Journal: PLoS One Date: 2014-09-24 Impact factor: 3.240
Authors: Victor Fabrizio Cabrera Pazmiño; Miguel Agusto Riquelme Rodas; Carlos David Barrios Cáceres; Guillermo Gustavo Renault Duarte; Melanie Vanesa Cano Azuaga; Bruna Luísa de Paula; Eliana Aparecida Caliente; Simone Soares; Elcia Maria Varize Silveira Journal: Case Rep Dent Date: 2017-06-13