| Literature DB >> 18753219 |
Takashi Kimura1,2, Michio Imamura1,2, Nobuhiko Hiraga1,2, Tsuyoshi Hatakeyama1,2, Daiki Miki1,2, Chiemi Noguchi1,2, Nami Mori1,2, Masataka Tsuge1,2, Shoichi Takahashi1,2, Yoshifumi Fujimoto1,2, Eiji Iwao3, Hidenori Ochi4,1, Hiromi Abe4,1,2, Toshiro Maekawa4, Keiko Arataki5, Chise Tateno6,1, Katsutoshi Yoshizato6,1, Takaji Wakita7, Toru Okamoto8, Yoshiharu Matsuura8, Kazuaki Chayama4,1,2.
Abstract
The establishment of clonal infection of hepatitis C virus (HCV) in a small-animal model is important for the analysis of HCV virology. A previous study developed models of molecularly cloned genotype 1a and 2a HCV infection using human hepatocyte-transplanted chimeric mice. This study developed a new model of molecularly cloned genotype 1b HCV infection. A full-length genotype 1b HCV genome, HCV-KT9, was cloned from a serum sample from a patient with severe acute hepatitis. The chimeric mice were inoculated intrahepatically with in vitro-transcribed HCV-KT9 RNA. Inoculated mice developed viraemia at 2 weeks post-infection, and this persisted for more than 6 weeks. Passage experiments indicated that the sera of these mice contained infectious HCV. Interestingly, a similar clone, HCV-KT1, in which the poly(U/UC) tract was 29 nt shorter than in HCV-KT9, showed poorer in vivo infectivity and replication ability. An in vitro study showed that no virus was produced in the culture medium from HCV-KT9-transfected cells. In conclusion, this study developed a genetically engineered genotype 1b HCV-infected mouse. This mouse model will be useful for the study of HCV virology, particularly the mechanism underlying the variable resistance of HCV genotypes to interferon therapy.Entities:
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Year: 2008 PMID： 18753219 DOI： 10.1099/vir.0.83658-0
Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891