M Costalonga1, L Batas, B J Reich. 1. Division of Periodontology, Department of Developmental and Surgical Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USA. costa002@umn.edu
Abstract
BACKGROUND AND OBJECTIVE: Toll-like receptor 4 (TLR-4)/myeloid differentiation protein-2 complex ligation by lipopolysaccharide induces production of pro-inflammatory cytokines and co-stimulatory molecules on antigen presenting cells. The aim of this study was to determine the role of the TLR-4 in bone loss-resistant C57BL mice and in bone loss-susceptible BALB/c mice after infection with Porphyromonas gingivalis. MATERIAL AND METHODS: The BALB/c and C57BL/10 mice, either normal or TLR-4 deficient, were infected or sham-infected orally four times, at 4 day intervals, with 10(9) colony forming units of P. gingivalis. At 47 days, defleshed jaws were stained and photographed in a standardized position. We measured the surface area of the root trunk to assess the alveolar bone loss. RESULTS: Porphyromonas gingivalis-infected wild-type BALB/c mice lost 13.8% more bone than P. gingivalis-infected wild-type C57BL/10 mice. In contrast, P. gingivalis-infected TLR-4-deficient C57BL/10 mice lost 12.7% more bone than P. gingivalis-infected TLR-4-deficient BALB/c mice. Porphyromonas gingivalis-infected wild-type C57BL/6 and TLR-2 knockout C57BL/6 mice had similar bone levels to sham-infected control mice. CONCLUSION: Toll-like receptor 4 is protective for C57BL/10 but detrimental to BALB/c mice, since its absence allowed C57BL/10 but not BALB/c mice to lose alveolar bone. Toll-like receptor 2 does not contribute to this protection in genetically similar C57BL/6 mice.
BACKGROUND AND OBJECTIVE:Toll-like receptor 4 (TLR-4)/myeloid differentiation protein-2 complex ligation by lipopolysaccharide induces production of pro-inflammatory cytokines and co-stimulatory molecules on antigen presenting cells. The aim of this study was to determine the role of the TLR-4 in bone loss-resistant C57BL mice and in bone loss-susceptible BALB/c mice after infection with Porphyromonas gingivalis. MATERIAL AND METHODS: The BALB/c and C57BL/10 mice, either normal or TLR-4 deficient, were infected or sham-infected orally four times, at 4 day intervals, with 10(9) colony forming units of P. gingivalis. At 47 days, defleshed jaws were stained and photographed in a standardized position. We measured the surface area of the root trunk to assess the alveolar bone loss. RESULTS:Porphyromonas gingivalis-infected wild-type BALB/c mice lost 13.8% more bone than P. gingivalis-infected wild-type C57BL/10 mice. In contrast, P. gingivalis-infectedTLR-4-deficient C57BL/10 mice lost 12.7% more bone than P. gingivalis-infectedTLR-4-deficient BALB/c mice. Porphyromonas gingivalis-infected wild-type C57BL/6 and TLR-2 knockout C57BL/6 mice had similar bone levels to sham-infected control mice. CONCLUSION:Toll-like receptor 4 is protective for C57BL/10 but detrimental to BALB/c mice, since its absence allowed C57BL/10 but not BALB/c mice to lose alveolar bone. Toll-like receptor 2 does not contribute to this protection in genetically similar C57BL/6 mice.
Authors: A Meka; V Bakthavatchalu; S Sathishkumar; M C Lopez; R K Verma; S M Wallet; I Bhattacharyya; B F Boyce; M Handfield; R J Lamont; H V Baker; J L Ebersole; L Kesavalu Journal: Mol Oral Microbiol Date: 2010-02 Impact factor: 3.563
Authors: Peter D Bittner-Eddy; Lori A Fischer; Daniel H Kaplan; Kathleen Thieu; Massimo Costalonga Journal: J Immunol Date: 2016-07-11 Impact factor: 5.422
Authors: G Papadopoulos; Y B Shaik-Dasthagirisaheb; N Huang; G A Viglianti; A J Henderson; A Kantarci; F C Gibson Journal: Mol Oral Microbiol Date: 2016-08-26 Impact factor: 3.563