Literature DB >> 1874890

Rapid high-performance liquid chromatographic assay for total homocysteine levels in human serum.

J B Ubbink1, W J Hayward Vermaak, S Bissbort.   

Abstract

A rapid, isocratic high-performance liquid chromatographic (HPLC) method is described for the determination of total homocysteine levels in human serum. Prior to reversed-phase HPLC analysis, the serum thiols were derivatized with SBD-F (ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate), a thiolspecific fluorogenic probe which is commercially available. Retention of SBD-homocysteine was sensitive to pH, and a mobile phase pH of 2.1 ensured baseline separation of serum thiols within 6 min. The method is simple, sensitive, reproducible (between-run coefficient of variation of 6.6%) and very suitable for routine determination of serum homocysteine levels in a clinical pathology laboratory.

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Year:  1991        PMID： 1874890     DOI： 10.1016/0378-4347(91)80407-4

Source DB:  PubMed          Journal:  J Chromatogr


  95 in total

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