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Literature DB >> 187343

Localization of K+-stimulated p-NPPase in the lachrymal 'salt' gland of Malaclemys, using cytochemical and autoradiographical techniques.

Abstract

Using two independent techniques, histochemistry and autoradiography, an enzyme (E.C. 3.6.1.3.) has been localized on basolateral cell membranes of salt secreting cells in the lachrymal gland of Malaclemys. This enzyme is ouabain sensitive. In addition an L-tetramisole sensitive alkaline phosphatase is found in the same sites, and an ethacrynic acid sensitive K+-stimulated p-NPPase is found on the apical membrane. The significance of these results with regard to the location of the pump responsible for net transepithelial sodium transport is discussed.

Entities: Chemical

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Year: 1976 PMID： 187343 DOI： 10.1007/bf00220684

Source DB: PubMed Journal: Cell Tissue Res ISSN： 0302-766X Impact factor: 5.249

12 in total

1. Transport adenosine triphosphatase cytochemistry. II. Cytochemical localization of ouabin-sensitive, potassium-dependent phosphatase activity in the secretory epithelium of the avian salt gland.

Authors: S A Ernst
Journal: J Histochem Cytochem Date: 1972-01 Impact factor: 2.479

2. Relation between cell Na extrusion and transtubular absorption in the perfused toad kidney: the effect of K, ouabain and ethacrynic acid.

Authors: G Whittembury; J Fishman
Journal: Pflugers Arch Date: 1969 Impact factor: 3.657

3. Problems associated with the demonstration by lead methods of adenosine triphosphatase activity in resident peritoneal macrophages and exudate monocytes of the guinea pig.

Authors: R E Poelmann; W T Daems
Journal: J Histochem Cytochem Date: 1973-05 Impact factor: 2.479

4. The homology of cranial glands in turtles: with special reference to the nomenclature of salt glands.

Authors: F B Cowan
Journal: J Morphol Date: 1973-10 Impact factor: 1.804

5. Some aspects of electrolyte and water balance in three estuarine reptiles, the diamondback terrapin, American and "salt water" crocodiles.

Authors: W A Dunson
Journal: Comp Biochem Physiol Date: 1970-01-15

6. The development of surface specialization in the secretory epithelium of the avian salt gland in response to osmotic stress.

Authors: S A Ernst; R A Ellis
Journal: J Cell Biol Date: 1969-02 Impact factor: 10.539

7. Observations on K+-stimulated p-nitrophenylphosphatase in the lachrymal gland of Malaclemys terrapin, during adaptation to salt and fresh water.

Authors: F B Cowan
Journal: Comp Biochem Physiol A Comp Physiol Date: 1974-12-01

2. Ultrahistochemical and autoradiographic evidence of epithelial transport in the uterus of the ovoviviparous salamander, Salamandra salamandra (L.) (Amphibia, Urodela).

Authors: H Greven
Journal: Cell Tissue Res Date: 1980 Impact factor: 5.249

2 in total

Localization of K+-stimulated p-NPPase in the lachrymal 'salt' gland of Malaclemys, using cytochemical and autoradiographical techniques.

1. Transport adenosine triphosphatase cytochemistry. II. Cytochemical localization of ouabin-sensitive, potassium-dependent phosphatase activity in the secretory epithelium of the avian salt gland.

2. Relation between cell Na extrusion and transtubular absorption in the perfused toad kidney: the effect of K, ouabain and ethacrynic acid.

3. Problems associated with the demonstration by lead methods of adenosine triphosphatase activity in resident peritoneal macrophages and exudate monocytes of the guinea pig.

4. The homology of cranial glands in turtles: with special reference to the nomenclature of salt glands.

5. Some aspects of electrolyte and water balance in three estuarine reptiles, the diamondback terrapin, American and "salt water" crocodiles.

6. The development of surface specialization in the secretory epithelium of the avian salt gland in response to osmotic stress.

7. Observations on K+-stimulated p-nitrophenylphosphatase in the lachrymal gland of Malaclemys terrapin, during adaptation to salt and fresh water.

8. Standing-gradient osmotic flow. A mechanism for coupling of water and solute transport in epithelia.

9. Localization of sodium pumps in the choroid plexus epithelium.

10. Radioautographic localization of sodium pump sites in rabbit intestine.

1. Adenosine triphosphatase localization in the branchial heart appendage of Sepia officinalis L. (Cephalopoda).

2. Ultrahistochemical and autoradiographic evidence of epithelial transport in the uterus of the ovoviviparous salamander, Salamandra salamandra (L.) (Amphibia, Urodela).