| Literature DB >> 18729059 |
Lindsay Davis1, Kathryn Young, Victor DiRita.
Abstract
Molecular manipulation has been a limiting factor in C. jejuni research for many years. Recent advances in molecular techniques adapted for C. jejuni have furthered our understanding of the organism. This unit is dedicated to common molecular tools in bacterial research specifically tailored for C. jejuni. These include colony PCR, DNA isolation, and RNA isolation. The unit also reviews techniques for genetic manipulation, such as the use of plasmids, natural transformation, electroporation, conjugation, and transposition. In addition, a reporter system, the arylsulfatase assay, can be used to study gene expression. Copyright 2008 by John Wiley & Sons, Inc.Entities:
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Year: 2008 PMID: 18729059 PMCID: PMC5147580 DOI: 10.1002/9780471729259.mc08a02s10
Source DB: PubMed Journal: Curr Protoc Microbiol ISSN: 1934-8525