Literature DB >> 1872850

Type II phospholipase A2 recombinant overexpression enhances stimulated arachidonic acid release.

P Pernas1, J Masliah, J L Olivier, C Salvat, T Rybkine, G Bereziat.   

Abstract

The coding sequence of type II phospholipase A2 from human placenta was cloned in a bovine papilloma virus-derived eukaryotic expression vector under the control of the metallothionein promoter. Stably transfected C127 mouse fibroblast lines were obtained with this vector. These transfected cells overexpressed a functional 14 kDa phospholipase A2, which was bulky secreted. However, a significant phospholipase A2 activity was measured in cell homogenates. The involvement of this 14 kDa phospholipase A2 in mechanisms related to stimulated arachidonic acid release was investigated. We could parallel the overexpression of phospholipase A2 with an increase in phorbol ester and fluoroaluminate-stimulated arachidonic acid release. Pertussis toxin inhibited this stimulation. These results suggest that the 14 kDa type II phospholipase A2 might contribute to stimulation of arachidonic acid release, and therefore to eicosanoid production.

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Year:  1991        PMID： 1872850     DOI： 10.1016/0006-291x(91)91035-b

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  14 in total

1.  Posttranscriptional effect of insulin-like growth factor-I on interleukin-1beta-induced type II-secreted phospholipase A2 gene expression in rabbit articular chondrocytes.

Authors:  C Jacques; G Béréziat; L Humbert; J L Olivier; M T Corvol; J Masliah; F Berenbaum
Journal:  J Clin Invest       Date:  1997-04-15       Impact factor: 14.808

2.  The localization of phospholipase A2 in the secretory granule.

Authors:  S P Chock; E A Schmauder-Chock; E Cordella-Miele; L Miele; A B Mukherjee
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857

3.  Modulation of human type II secretory phospholipase A2 by sphingomyelin and annexin VI.

Authors:  K Koumanov; C Wolf; G Béreziat
Journal:  Biochem J       Date:  1997-08-15       Impact factor: 3.857

4.  Regulation of arachidonic acid, eicosanoid, and phospholipase A2 levels in murine mast cells by recombinant stem cell factor.

Authors:  A N Fonteh; J M Samet; F H Chilton
Journal:  J Clin Invest       Date:  1995-09       Impact factor: 14.808

5.  Cholesterol relieves the inhibitory effect of sphingomyelin on type II secretory phospholipase A2.

Authors:  K S Koumanov; P J Quinn; G Béréziat; C Wolf
Journal:  Biochem J       Date:  1998-12-15       Impact factor: 3.857

6.  Cytokine-stimulated secretion of group II phospholipase A2 by rat mesangial cells. Its contribution to arachidonic acid release and prostaglandin synthesis by cultured rat glomerular cells.

Authors:  J Pfeilschifter; C Schalkwijk; V A Briner; H van den Bosch
Journal:  J Clin Invest       Date:  1993-11       Impact factor: 14.808

7.  Arachidonic acid mobilization in P388D1 macrophages is controlled by two distinct Ca(2+)-dependent phospholipase A2 enzymes.

Authors:  J Balsinde; S E Barbour; I D Bianco; E A Dennis
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-08       Impact factor: 11.205

8.  Suppression of endothelin-1-induced mitogenic responses of human aortic smooth muscle cells by interleukin-1 beta.

Authors:  Y Fujitani; H Ninomiya; T Okada; Y Urade; T Masaki
Journal:  J Clin Invest       Date:  1995-06       Impact factor: 14.808

9.  The requirement for phospholipase A2 for activation of the assembled NADPH oxidase in human neutrophils.

Authors:  R Dana; H L Malech; R Levy
Journal:  Biochem J       Date:  1994-01-01       Impact factor: 3.857

10.  Sequence specific inhibition of human type II phospholipase A2 enzyme activity by phosphorothioate oligonucleotides.

Authors:  C F Bennett; M Y Chiang; L Wilson-Lingardo; J R Wyatt
Journal:  Nucleic Acids Res       Date:  1994-08-11       Impact factor: 16.971
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