Literature DB >> 18726472

T7 RNA polymerase transcription of Escherichia coli isoacceptors tRNA(Leu).

Y Li1, J Chen, E Wang, Y Wang.   

Abstract

The T7 promoter and the gene encoding tRNA(Leu) were synthesized chemically and cloned into plasmid pUC19, where the gene was transcribed in vitro with T7 RNA polymerase purified. It was found that spermidine had a negative effect on T7 transcription. The amount of transcribed tRNA was about 250 times that of the template DNA under optimal reaction conditions. The unmodified tRNA(1) (Leu) and tRNA(2) (Leu) had similar leucine accepting ability, which however was only one fourth that of the native modified ones. This result indicated that the modified nucleotides of tRNA(Leu) played some though not key roles in its aminoacylation.

Entities:  

Year:  1999        PMID： 18726472     DOI： 10.1007/BF02880055

Source DB:  PubMed          Journal:  Sci China C Life Sci        ISSN： 1006-9305


  19 in total

1.  Tertiary structure base pairs between D- and TpsiC-loops of Escherichia coli tRNA(Leu) play important roles in both aminoacylation and editing.

Authors:  Xing Du; En-Duo Wang
Journal:  Nucleic Acids Res       Date:  2003-06-01       Impact factor: 16.971

2.  The CP2 domain of leucyl-tRNA synthetase is crucial for amino acid activation and post-transfer editing.

Authors:  Xiao-Long Zhou; Bin Zhu; En-Duo Wang
Journal:  J Biol Chem       Date:  2008-10-27       Impact factor: 5.157

3.  A hypertension-associated mitochondrial DNA mutation alters the tertiary interaction and function of tRNALeu(UUR).

Authors:  Mi Zhou; Meng Wang; Ling Xue; Zhi Lin; Qiufen He; Wenwen Shi; Yaru Chen; Xiaofen Jin; Haiying Li; Pingping Jiang; Min-Xin Guan
Journal:  J Biol Chem       Date:  2017-07-05       Impact factor: 5.157

4.  Sequence-specific and Shape-selective RNA Recognition by the Human RNA 5-Methylcytosine Methyltransferase NSun6.

Authors:  Tao Long; Jing Li; Hao Li; Mi Zhou; Xiao-Long Zhou; Ru-Juan Liu; En-Duo Wang
Journal:  J Biol Chem       Date:  2016-10-04       Impact factor: 5.157

5.  A hypertension-associated mitochondrial DNA mutation introduces an m1G37 modification into tRNAMet, altering its structure and function.

Authors:  Mi Zhou; Ling Xue; Yaru Chen; Haiying Li; Qiufen He; Bibin Wang; Feilong Meng; Meng Wang; Min-Xin Guan
Journal:  J Biol Chem       Date:  2017-12-08       Impact factor: 5.157

6.  Contribution of a mitochondrial tyrosyl-tRNA synthetase mutation to the phenotypic expression of the deafness-associated tRNASer(UCN) 7511A>G mutation.

Authors:  Wenlu Fan; Jing Zheng; Wanzhong Kong; Limei Cui; Maerhaba Aishanjiang; Qiuzi Yi; Min Wang; Xiaohui Cang; Xiaowen Tang; Ye Chen; Jun Qin Mo; Neal Sondheimer; Wanzhong Ge; Min-Xin Guan
Journal:  J Biol Chem       Date:  2019-11-04       Impact factor: 5.157

7.  Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.

Authors:  Shasha Gong; Xiaoqiong Wang; Feilong Meng; Limei Cui; Qiuzi Yi; Qiong Zhao; Xiaohui Cang; Zhiyi Cai; Jun Qin Mo; Yong Liang; Min-Xin Guan
Journal:  J Biol Chem       Date:  2019-12-09       Impact factor: 5.157

Review 8.  Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System.

Authors:  Jiaojiao Li; Mengtong Tang; Hao Qi
Journal:  Front Bioeng Biotechnol       Date:  2022-05-13

9.  Heteroplasmic and homoplasmic m.616T>C in mitochondria tRNAPhe promote isolated chronic kidney disease and hyperuricemia.

Authors:  Chengxian Xu; Lingxiao Tong; Jia Rao; Qing Ye; Yuxia Chen; Yingying Zhang; Jie Xu; Xiaoting Mao; Feilong Meng; Huijun Shen; Zhihong Lu; Xiaohui Cang; Haidong Fu; Shugang Wang; Weiyue Gu; En-Yin Lai; Min-Xin Guan; Pingping Jiang; Jianhua Mao
Journal:  JCI Insight       Date:  2022-06-08

10.  In vivo identification of essential nucleotides in tRNALeu to its functions by using a constructed yeast tRNALeu knockout strain.

Authors:  Qian Huang; Peng Yao; Gilbert Eriani; En-Duo Wang
Journal:  Nucleic Acids Res       Date:  2012-08-23       Impact factor: 16.971
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