Literature DB >> 1872473

Assay of HIV-1 proteinase: a colorimetric method using small peptide substrates.

A V Broadhurst1, N A Roberts, A J Ritchie, B K Handa, C Kay.   

Abstract

A colorimetric assay for HIV proteinase using small protected peptide substrates is described. Substrates are cleaved to release N-terminal prolyl peptides which react with isatin to form a blue product which is measured spectrophotometrically. The assay is suitable for use with pure enzyme or crude extracts derived from genetically engineered Escherichia coli.

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Year:  1991        PMID： 1872473     DOI： 10.1016/0003-2697(91)90022-l

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  2 in total

1.  Kinetics and mechanism of autoprocessing of human immunodeficiency virus type 1 protease from an analog of the Gag-Pol polyprotein.

Authors:  J M Louis; N T Nashed; K D Parris; A R Kimmel; D M Jerina
Journal:  Proc Natl Acad Sci U S A       Date:  1994-08-16       Impact factor: 11.205

2.  Synthesis and antibacterial activities of novel 2,5-diphenylindolo[2,3-e] pyrazolo[1',5':3",4"]pyrimido[2",1"-c] [1,2,4]triazines.

Authors:  Kamal F M Atta; Omaima O M Farahat; Somaya M Ghobashy; Mohamed G Marei
Journal:  Molecules       Date:  2011-12-15       Impact factor: 4.411
  2 in total

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