Zhi-Feng Yan1, Ben-Gang Zhang, Zhao Zhang. 1. Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100094, China.
Abstract
OBJECTIVE: To evaluate the genetic diversity from ex-situ conservation population of Phellodendron amurense. METHOD: Genetic diversity in 67 individuals from ex-situ conservation population of P. amurense were assessed using AFLP technique by eight fluorescent labeled primer groups. RESULT: The eight primer combinations generated a total of 1,574 bands, of which 1,354 were polymorphic. As for P. amurense at species level, the percentage of polymorphic loci (PPL) was 86.02, the effective number of alleles per locus (Ne) was 1.4275 ,the Nei's gene diversity (H) was 0.2312, and the Shannon's information index (I) was 0.3973. At the population level, the estimates PPL=41.55, H=0.1400, Ne=0.2376 and I=0.2113. CONCLUSION: The effect of present ex-situ conservation was preliminarily assessed.
OBJECTIVE: To evaluate the genetic diversity from ex-situ conservation population of Phellodendron amurense. METHOD: Genetic diversity in 67 individuals from ex-situ conservation population of P. amurense were assessed using AFLP technique by eight fluorescent labeled primer groups. RESULT: The eight primer combinations generated a total of 1,574 bands, of which 1,354 were polymorphic. As for P. amurense at species level, the percentage of polymorphic loci (PPL) was 86.02, the effective number of alleles per locus (Ne) was 1.4275 ,the Nei's gene diversity (H) was 0.2312, and the Shannon's information index (I) was 0.3973. At the population level, the estimates PPL=41.55, H=0.1400, Ne=0.2376 and I=0.2113. CONCLUSION: The effect of present ex-situ conservation was preliminarily assessed.