Literature DB >> 18716860

Exploring the nature of photo-damage in two-photon excitation by fluorescence intensity modulation.

Arijit Kumar De1, Debabrata Goswami.   

Abstract

We investigate the relative photo-damage effects during one- and two-photon excitations and demonstrate that there exist fundamental differences in the damage induced by a high repetition rate laser as compared to that of a CW laser. This difference is evident from the degree of enhanced fluorescence intensity achieved by blanking the excitation with an optical chopper. Such an enhancement in fluorescence intensity provides better signal-to-noise ratio that could have immediate applications in multiphoton imaging of live specimens.

Mesh：

Year:  2008        PMID： 18716860     DOI： 10.1007/s10895-008-0405-3

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  9 in total

1.  Highly nonlinear photodamage in two-photon fluorescence microscopy.

Authors:  A Hopt; E Neher
Journal:  Biophys J       Date:  2001-04       Impact factor: 4.033

2.  Photobleaching in two-photon excitation microscopy.

Authors:  G H Patterson; D W Piston
Journal:  Biophys J       Date:  2000-04       Impact factor: 4.033

3.  Two-photon thermal bleaching of single fluorescent molecules.

Authors:  Giuseppe Chirico; Fabio Cannone; Giancarlo Baldini; Alberto Diaspro
Journal:  Biophys J       Date:  2003-01       Impact factor: 4.033

4.  Evaluation of single-photon-counting measurements of excited-state lifetimes.

Authors:  H E Zimmerman; J H Penn; C W Carpenter
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

5.  Ultrafast measurement of two-photon absorption by loss modulation.

Authors:  Peifang Tian; Warren S Warren
Journal:  Opt Lett       Date:  2002-09-15       Impact factor: 3.776

6.  Analysis of efficiency of two-photon versus single-photon absorption of fluorescence generation in biological objects.

Authors:  G J Brakenhoff; M Müller; R I Ghauharali
Journal:  J Microsc       Date:  1996-08       Impact factor: 1.758

Review 7.  Photon upmanship: why multiphoton imaging is more than a gimmick.

Authors:  W Denk; K Svoboda
Journal:  Neuron       Date:  1997-03       Impact factor: 17.173

8.  Two-photon laser scanning fluorescence microscopy.

Authors:  W Denk; J H Strickler; W W Webb
Journal:  Science       Date:  1990-04-06       Impact factor: 47.728

9.  Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation: signal and photodamage.

Authors:  H J Koester; D Baur; R Uhl; S W Hell
Journal:  Biophys J       Date:  1999-10       Impact factor: 4.033
  9 in total
  9 in total

1.  Concentration Dependent Approach for Accurate Determination of Two-Photon Absorption Cross-Section of Fluorescent dye Molecule.

Authors:  Sandeep Kumar Maurya; Chayan Dutta; Debabrata Goswami
Journal:  J Fluoresc       Date:  2017-04-11       Impact factor: 2.217

2.  A systematic study on fluorescence enhancement under single-photon pulsed illumination.

Authors:  Arijit Kumar De; Debabrata Goswami
Journal:  J Fluoresc       Date:  2009-06-09       Impact factor: 2.217

3.  Two-stage deep learning network-based few-view image reconstruction for parallel-beam projection tomography.

Authors:  Huiyuan Wang; Nan Wang; Hui Xie; Lin Wang; Wangting Zhou; Defu Yang; Xu Cao; Shouping Zhu; Jimin Liang; Xueli Chen
Journal:  Quant Imaging Med Surg       Date:  2022-04

4.  Towards controlling molecular motions in fluorescence microscopy and optical trapping: a spatiotemporal approach.

Authors:  Arijit Kumar De; Debabrata Goswami
Journal:  Int Rev Phys Chem       Date:  2011-09-26       Impact factor: 4.762

5.  Coherent Control in Multiphoton Fluorescence Imaging.

Authors:  Arijit Kumar De; Debabrata Goswami
Journal:  Proc SPIE Int Soc Opt Eng       Date:  2009-02-25

6.  Three-dimensional image formation under single-photon ultra-short pulsed illumination.

Authors:  Arijit Kumar De; Debabrata Goswami
Journal:  Proc SPIE Int Soc Opt Eng       Date:  2009-05-22

7.  Correcting for photodestruction in super-resolution optical fluctuation imaging.

Authors:  Yves Peeters; Wim Vandenberg; Sam Duwé; Arno Bouwens; Tomáš Lukeš; Cyril Ruckebusch; Theo Lasser; Peter Dedecker
Journal:  Sci Rep       Date:  2017-09-05       Impact factor: 4.379

8.  Nonlinear Two-Photon Absorption in the Near-Infrared Band for Lead Bromide Perovskite Films Using an F-Scan Nonlinear Spectrometer.

Authors:  Edgar Rueda; Juan Serna; Jose Ignacio Uribe; Daniel Ramírez; Franklin Jaramillo; Jaime Osorio; Hernando García
Journal:  ACS Omega       Date:  2022-08-09

9.  Solvent effect on two-photon absorption and fluorescence of rhodamine dyes.

Authors:  Amit Nag; Debabrata Goswami
Journal:  J Photochem Photobiol A Chem       Date:  2009-08-15       Impact factor: 4.291
  9 in total

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