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Literature DB >> 18713621

Simplified purification procedure of laminin-332 and laminin-511 from human cell lines.

Isis C Sroka¹, Man Ling Chen, Anne E Cress.

Abstract

Laminins are glycoproteins expressed in the basement membrane of multiple epithelial tissues. Previously described purification procedures for the human laminin variants laminin-5 (LN-332) and laminin-10 (LN-511) use tissue as starting material and have multiple steps. We demonstrate a two-step laminin immunoaffinity purification method to produce consistent quantities of intact and biologically active LN-332 and LN-511 from human keratinocyte (HaCaT) and human lung carcinoma (A549) cell lines, respectively. The purification of LN-332 and LN-551 was demonstrated by PAGE analysis, silver staining and Western blot analysis. The purification procedure includes instruction on removing a cell adhesion contaminant known as galectin-3 binding protein from purified LN-511. The biological activity of purified laminin was tested in a standard cell adhesion assay and compared to commercially available LN-111. This rapid and reproducible purification method will contribute to understanding the role of LN-332 and LN-511 in cell behavior, signaling, and gene expression.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：

Year: 2008 PMID： 18713621 PMCID： PMC2573946 DOI： 10.1016/j.bbrc.2008.08.029

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

19 in total

Review 1. Laminin functions in tissue morphogenesis.

Authors: Jeffrey H Miner; Peter D Yurchenco
Journal: Annu Rev Cell Dev Biol Date: 2004 Impact factor: 13.827

2. Recombinant human laminin-10 (alpha5beta1gamma1). Production, purification, and migration-promoting activity on vascular endothelial cells.

Authors: Masayuki Doi; Jill Thyboll; Jarkko Kortesmaa; Katarina Jansson; Antti Iivanainen; Masomeh Parvardeh; Rupert Timpl; Ulf Hedin; Jesper Swedenborg; Karl Tryggvason
Journal: J Biol Chem Date: 2002-01-30 Impact factor: 5.157

3. Herlitz's junctional epidermolysis bullosa is linked to mutations in the gene (LAMC2) for the gamma 2 subunit of nicein/kalinin (LAMININ-5).

Authors: D Aberdam; M F Galliano; J Vailly; L Pulkkinen; J Bonifas; A M Christiano; K Tryggvason; J Uitto; E H Epstein; J P Ortonne
Journal: Nat Genet Date: 1994-03 Impact factor: 38.330

4. Membrane type-1-matrix metalloproteinase expressed by prostate carcinoma cells cleaves human laminin-5 beta3 chain and induces cell migration.

Authors: Thirupandiyur S Udayakumar; Man Ling Chen; Elisabeth L Bair; Dorothea C Von Bredow; Anne E Cress; Raymond B Nagle; G Timothy Bowden
Journal: Cancer Res Date: 2003-05-01 Impact factor: 12.701

5. Mac-2-binding glycoproteins. Putative ligands for a cytosolic beta-galactoside lectin.

Authors: I Rosenberg; B J Cherayil; K J Isselbacher; S Pillai
Journal: J Biol Chem Date: 1991-10-05 Impact factor: 5.157

6. Integrin alpha 6 expression in human prostate carcinoma cells is associated with a migratory and invasive phenotype in vitro and in vivo.

Authors: I Rabinovitz; R B Nagle; A E Cress
Journal: Clin Exp Metastasis Date: 1995-11 Impact factor: 5.150

6. Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8.

Authors: Hitomi Fujisaki; Sugiko Futaki; Masashi Yamada; Kiyotoshi Sekiguchi; Toshihiko Hayashi; Takashi Ikejima; Shunji Hattori
Journal: Regen Ther Date: 2018-05-24 Impact factor: 3.419

7. CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition.

Authors: L Shaw; R L Williams; K J Hamill
Journal: BMC Res Notes Date: 2020-02-21

7 in total

Simplified purification procedure of laminin-332 and laminin-511 from human cell lines.

Review 1. Laminin functions in tissue morphogenesis.

2. Recombinant human laminin-10 (alpha5beta1gamma1). Production, purification, and migration-promoting activity on vascular endothelial cells.

3. Herlitz's junctional epidermolysis bullosa is linked to mutations in the gene (LAMC2) for the gamma 2 subunit of nicein/kalinin (LAMININ-5).

4. Membrane type-1-matrix metalloproteinase expressed by prostate carcinoma cells cleaves human laminin-5 beta3 chain and induces cell migration.

5. Mac-2-binding glycoproteins. Putative ligands for a cytosolic beta-galactoside lectin.

6. Integrin alpha 6 expression in human prostate carcinoma cells is associated with a migratory and invasive phenotype in vitro and in vivo.

7. A large cell-adhesive scatter factor secreted by human gastric carcinoma cells.

8. Expression of hemidesmosomal and extracellular matrix proteins by normal and malignant human prostate tissue.

9. Kalinin: an epithelium-specific basement membrane adhesion molecule that is a component of anchoring filaments.

10. Role of cell surface metalloprotease MT1-MMP in epithelial cell migration over laminin-5.

Review 1. The laminin binding integrin alpha6beta1 in prostate cancer perineural invasion.

2. Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells.

3. Characterization of Laminin Binding Integrin Internalization in Prostate Cancer Cells.

4. Targeting integrin α6 stimulates curative-type bone metastasis lesions in a xenograft model.

Review 5. Laminin-511: a multi-functional adhesion protein regulating cell migration, tumor invasion and metastasis.

6. Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8.

7. CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition.