Bo Kong1, Ying-long Liu, Xiao-dong Lü. 1. Pediatric Cardiac Center, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China. kongbo1980@yahoo.com.cn
Abstract
BACKGROUND: The physiological differences between fetal and postnatal heart have been well characterized at the cellular level. However, the genetic mechanisms governing and regulating these differences have only been partially elucidated. Elucidation of the differentially expressed genes profile before and after birth has never been systematically proposed and analyzed. METHODS: The human oligonucleotide microarray and bioinformatics analysis approaches were applied to isolate and classify the differentially expressed genes between fetal and infant cardiac tissue samples. Quantitative real-time PCR was used to confirm the results from the microarray. RESULTS: Two hundred and forty-two differentially expressed genes were discovered and classified into 13 categories, including genes related to energy metabolism, myocyte hyperplasia, development, muscle contraction, protein synthesis and degradation, extracellular matrix components, transcription factors, apoptosis, signal pathway molecules, organelle organization and several other biological processes. Moreover, 95 genes were identified which had not previously been reported to be expressed in the heart. CONCLUSIONS: The study systematically analyzed the alteration of the gene expression profile between the human fetal and infant myocardium. A number of genes were discovered which had not been reported to be expressed in the heart. The data provided insight into the physical development mechanisms of the heart before and after birth.
BACKGROUND: The physiological differences between fetal and postnatal heart have been well characterized at the cellular level. However, the genetic mechanisms governing and regulating these differences have only been partially elucidated. Elucidation of the differentially expressed genes profile before and after birth has never been systematically proposed and analyzed. METHODS: The human oligonucleotide microarray and bioinformatics analysis approaches were applied to isolate and classify the differentially expressed genes between fetal and infant cardiac tissue samples. Quantitative real-time PCR was used to confirm the results from the microarray. RESULTS: Two hundred and forty-two differentially expressed genes were discovered and classified into 13 categories, including genes related to energy metabolism, myocyte hyperplasia, development, muscle contraction, protein synthesis and degradation, extracellular matrix components, transcription factors, apoptosis, signal pathway molecules, organelle organization and several other biological processes. Moreover, 95 genes were identified which had not previously been reported to be expressed in the heart. CONCLUSIONS: The study systematically analyzed the alteration of the gene expression profile between the human fetal and infant myocardium. A number of genes were discovered which had not been reported to be expressed in the heart. The data provided insight into the physical development mechanisms of the heart before and after birth.
Authors: Elisabeth E Mlynarski; Molly B Sheridan; Michael Xie; Tingwei Guo; Silvia E Racedo; Donna M McDonald-McGinn; Xiaowu Gai; Eva W C Chow; Jacob Vorstman; Ann Swillen; Koen Devriendt; Jeroen Breckpot; Maria Cristina Digilio; Bruno Marino; Bruno Dallapiccola; Nicole Philip; Tony J Simon; Amy E Roberts; Małgorzata Piotrowicz; Carrie E Bearden; Stephan Eliez; Doron Gothelf; Karlene Coleman; Wendy R Kates; Marcella Devoto; Elaine Zackai; Damian Heine-Suñer; Tamim H Shaikh; Anne S Bassett; Elizabeth Goldmuntz; Bernice E Morrow; Beverly S Emanuel Journal: Am J Hum Genet Date: 2015-04-16 Impact factor: 11.025