Literature DB >> 18712524

Use of SSR markers to determine the anther-derived homozygous lines in coconut.

P I P Perera1, L Perera, V Hocher, J-L Verdeil, D M D Yakandawala, L K Weerakoon.   

Abstract

Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18712524     DOI： 10.1007/s00299-008-0592-z

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  3 in total

1.  Evaluating genetic relationships between indigenous coconut (Cocos nucifera L.) accessions from Sri Lanka by means of AFLP profiling.

Authors:  L Perera; J R Russell; J Provan; J W McNicol; W Powell
Journal:  Theor Appl Genet       Date:  1998-03       Impact factor: 5.699

2.  Isolation and characterization of polymorphic microsatellites in Cocos nucifera L.

Authors:  R Rivera; K J Edwards; J H Barker; G M Arnold; G Ayad; T Hodgkin; A Karp
Journal:  Genome       Date:  1999-08       Impact factor: 2.166

3.  Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria).

Authors:  Zoë Kernan; A M R Ferrie
Journal:  Plant Cell Rep       Date:  2005-10-18       Impact factor: 4.570
  3 in total
  4 in total

1.  Use of Molecular Markers for Doubled Haploid Technology: From Academia to Plant Breeding Companies.

Authors:  Stine Due Tuvesson; Clas-Tomas Larsson; Frank Ordon
Journal:  Methods Mol Biol       Date:  2021

Review 2.  Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review.

Authors:  Quang Thien Nguyen; H D Dharshani Bandupriya; Arturo López-Villalobos; S Sisunandar; Mike Foale; Steve W Adkins
Journal:  Planta       Date:  2015-07-19       Impact factor: 4.116

3.  A milestone in the doubled haploid pathway of cassava: a milestone in the doubled haploid pathway of cassava (Manihot esculenta Crantz): cellular and molecular assessment of anther-derived structures.

Authors:  P I P Perera; C A Ordoñez; L A Becerra Lopez-Lavalle; B Dedicova
Journal:  Protoplasma       Date:  2013-09-13       Impact factor: 3.356

4.  De novo transcriptome sequence assembly from coconut leaves and seeds with a focus on factors involved in RNA-directed DNA methylation.

Authors:  Ya-Yi Huang; Chueh-Pai Lee; Jason L Fu; Bill Chia-Han Chang; Antonius J M Matzke; Marjori Matzke
Journal:  G3 (Bethesda)       Date:  2014-09-04       Impact factor: 3.154
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.