OBJECTIVE: To examine bladder function in a newly developed experimental autoimmune cystitis (EAC) model in female SWXJ strain mice, as a potential animal model for interstitial cystitis (IC). MATERIALS AND METHODS: In all, 20 SWXJ female mice were divided into two groups: an EAC group immunized with mouse bladder homogenate in complete Freund's adjuvant (CFA) and a control group immunized with CFA alone. At 4 months after injection, the bladder function of some mice (six) was studied with 24-h micturition habits using metabolic cages and conscious cystometrography (CMG). The bladder and lung were harvested for histological examination and to assess interferon-gamma (IFN-gamma) mRNA expression. RESULTS: Histology examination showed obviously thickened lamina propria, infiltration of lymphocytes, giant cells, and increased mast cells in the detrusor muscle of the EAC mice. The lungs of EAC mice showed normal histology. The IFN-gamma mRNA expression increased significantly in the bladder, but not in the lung of the EAC mice. The 24-h micturition habits measurements showed increased frequency of urination in the EAC mice compared with the controls. Similarly, CMG showed decreased intercontraction intervals and voided volumes per micturition in the EAC mice compared with the controls. However, there were no significant differences in peak voiding pressure or total voiding volume between the EAC and control mice. CONCLUSIONS: Our murine EAC model has comparable functional and histological alterations to those seen in human IC, and may provide a useful model for the study of the pathogenesis and treatment of IC.
OBJECTIVE: To examine bladder function in a newly developed experimental autoimmune cystitis (EAC) model in female SWXJ strain mice, as a potential animal model for interstitial cystitis (IC). MATERIALS AND METHODS: In all, 20 SWXJ female mice were divided into two groups: an EAC group immunized with mouse bladder homogenate in complete Freund's adjuvant (CFA) and a control group immunized with CFA alone. At 4 months after injection, the bladder function of some mice (six) was studied with 24-h micturition habits using metabolic cages and conscious cystometrography (CMG). The bladder and lung were harvested for histological examination and to assess interferon-gamma (IFN-gamma) mRNA expression. RESULTS: Histology examination showed obviously thickened lamina propria, infiltration of lymphocytes, giant cells, and increased mast cells in the detrusor muscle of the EAC mice. The lungs of EAC mice showed normal histology. The IFN-gamma mRNA expression increased significantly in the bladder, but not in the lung of the EAC mice. The 24-h micturition habits measurements showed increased frequency of urination in the EAC mice compared with the controls. Similarly, CMG showed decreased intercontraction intervals and voided volumes per micturition in the EAC mice compared with the controls. However, there were no significant differences in peak voiding pressure or total voiding volume between the EAC and control mice. CONCLUSIONS: Our murine EAC model has comparable functional and histological alterations to those seen in human IC, and may provide a useful model for the study of the pathogenesis and treatment of IC.
Authors: Guiming Liu; Rania A Elrashidy; Nan Xiao; Michael Kavran; Yexiang Huang; Mingfang Tao; C Thomas Powell; Edward Kim; Ghazal Sadeghi; Hoda E Mohamed; Firouz Daneshgari Journal: Am J Physiol Cell Physiol Date: 2015-05-06 Impact factor: 4.249
Authors: Cengiz Z Altuntas; Firouz Daneshgari; Cagri Sakalar; Esen Goksoy; M Fatih Gulen; Michael Kavran; Jun Qin; Xiaoxia Li; Vincent K Tuohy Journal: Eur Urol Date: 2011-06-29 Impact factor: 20.096
Authors: Xiangrong Cui; Xuan Jing; Susan K Lutgendorf; Catherine S Bradley; Andrew Schrepf; Bradley A Erickson; Vincent A Magnotta; Timothy J Ness; Karl J Kreder; Michael A O'Donnell; Yi Luo Journal: Am J Physiol Renal Physiol Date: 2019-05-15