Literature DB >> 1870987

PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.

M C Fung1, K Y Fung.   

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Year:  1991        PMID： 1870987      PMCID： PMC328586          DOI： 10.1093/nar/19.15.4300

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


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  5 in total

1.  Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.

Authors:  C A Brenner; A W Tam; P A Nelson; E G Engleman; N Suzuki; K E Fry; J W Larrick
Journal:  Biotechniques       Date:  1989 Nov-Dec       Impact factor: 1.993

2.  The genes for leukemia inhibitory factor and interleukin-6 are expressed in mouse blastocysts prior to the onset of hemopoiesis.

Authors:  R Murray; F Lee; C P Chiu
Journal:  Mol Cell Biol       Date:  1990-09       Impact factor: 4.272

3.  Preparation of crude cell extract suitable for amplification of RNA by the polymerase chain reaction.

Authors:  F Ferre; F Garduno
Journal:  Nucleic Acids Res       Date:  1989-03-11       Impact factor: 16.971

4.  Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping.

Authors:  D A Rappolee; D Mark; M J Banda; Z Werb
Journal:  Science       Date:  1988-08-05       Impact factor: 47.728

5.  Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos.

Authors:  D A Rappolee; C A Brenner; R Schultz; D Mark; Z Werb
Journal:  Science       Date:  1988-09-30       Impact factor: 47.728
  5 in total
  3 in total

1.  RT-PCR with affinity-captured mRNA.

Authors:  K Cuddy; B Foley; J S Jaffe; D Gillespie
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

2.  Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells.

Authors:  G E Sheridan; C I Masters; J A Shallcross; B M MacKey
Journal:  Appl Environ Microbiol       Date:  1998-04       Impact factor: 4.792

3.  Overexpression and characterization of thermostable serine protease in Escherichia coli encoded by the ORF TTE0824 from Thermoanaerobacter tengcongensis.

Authors:  D Koma; H Yamanaka; K Moriyoshi; T Ohmoto; K Sakai
Journal:  Extremophiles       Date:  2007-07-27       Impact factor: 2.395
  3 in total

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