BACKGROUND: Endobronchial forceps biopsies are often small and are associated with a relevant extent of artifacts. To overcome these limitations is an important task. Especially when considering predictive factors for pharmacological therapies of lung cancer (ERCC1, RRM1) a development of biopsy techniques seems to be essential. This is the first report on a new endobronchial biopsy technique called cryobiopsy. OBJECTIVES: In this study the feasibility and the potential advantages of applying cryoprobes for harvesting samples for histological examination in flexible bronchoscopies will be focused on. METHODS: In 12 patients suffering from exophytic endobronchial malignancies, a modified flexible cryoprobe was used for immediate recanalization. The extracted tissue was examined histologically regarding sample quality and sample size. RESULTS: Tissue samples obtained using the cryoprobe showed an extraordinary good quality in terms of size (median diameter of 6.7 mm, range 4.2-13 mm) and artifact-free sample area (75% of the samples showed an artifact-free sample area of more than 75%). Additionally molecular markers were shown to be well preserved. CONCLUSIONS: The new technique termed cryobiopsy might widen the chest physician's range of tools for diagnostic bronchoscopies. Copyright 2008 S. Karger AG, Basel.
BACKGROUND: Endobronchial forceps biopsies are often small and are associated with a relevant extent of artifacts. To overcome these limitations is an important task. Especially when considering predictive factors for pharmacological therapies of lung cancer (ERCC1, RRM1) a development of biopsy techniques seems to be essential. This is the first report on a new endobronchial biopsy technique called cryobiopsy. OBJECTIVES: In this study the feasibility and the potential advantages of applying cryoprobes for harvesting samples for histological examination in flexible bronchoscopies will be focused on. METHODS: In 12 patients suffering from exophytic endobronchial malignancies, a modified flexible cryoprobe was used for immediate recanalization. The extracted tissue was examined histologically regarding sample quality and sample size. RESULTS: Tissue samples obtained using the cryoprobe showed an extraordinary good quality in terms of size (median diameter of 6.7 mm, range 4.2-13 mm) and artifact-free sample area (75% of the samples showed an artifact-free sample area of more than 75%). Additionally molecular markers were shown to be well preserved. CONCLUSIONS: The new technique termed cryobiopsy might widen the chest physician's range of tools for diagnostic bronchoscopies. Copyright 2008 S. Karger AG, Basel.
Authors: Karl-Josef Franke; Georg Nilius; Karl-Heinz Ruehle; Markus D Enderle; Walter Linzenbold; Claus Hann von Weyhern Journal: Lung Date: 2013-08-30 Impact factor: 2.584
Authors: Karl-Josef Franke; Mara Szyrach; Georg Nilius; Jürgen Hetzel; Martin Hetzel; Karl-Heinz Ruehle; Markus D Enderle Journal: Lung Date: 2009-06-23 Impact factor: 2.584
Authors: Anuradha Ramaswamy; Robert Homer; Jonathan Killam; Margaret A Pisani; Terrence E Murphy; Katy Araujo; Jonathan Puchalski Journal: J Bronchology Interv Pulmonol Date: 2016-01