Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Generation and characterization of novel monoclonal antibodies against human aurora-A.

Literature DB >> 18707550

Generation and characterization of novel monoclonal antibodies against human aurora-A.

Abstract

The mitotic kinase Aurora-A is essential for mitotic progression, including centrosome maturation, mitotic spindle formation, and faithful segregation of chromosomes to daughter cells. Several lines of evidences also suggest that the mammalian aurora kinase family proteins play a role in tumorigenesis. We have previously shown that human Aurora-A was ubiquitinated and negatively regulated by an early mitotic checkpoint protein, Chfr (checkpoint protein with FHA and RING domain). Here, we established several mouse anti-Aurora-A monoclonal antibodies (MAb). GST-tagged human Aurora-A was expressed in BL21 and used as an antigen to immunize mice. Three different hybridomas were obtained and antibodies produced by these hybridomas were analyzed. The results reveal that these antibodies specifically recognize endogenous Aurora-A in both immunoblotting and immunofluroscence experiments. They are useful tools for further analysis of human Aurora-A.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 2008 PMID： 18707550 PMCID： PMC3186696 DOI： 10.1089/hyb.2008.0016

Source DB: PubMed Journal: Hybridoma (Larchmt) ISSN： 1554-0014

14 in total

1. MLN8054, a small-molecule inhibitor of Aurora A, causes spindle pole and chromosome congression defects leading to aneuploidy.

Authors: Kara Hoar; Arijit Chakravarty; Claudia Rabino; Deborah Wysong; Douglas Bowman; Natalie Roy; Jeffrey A Ecsedy
Journal: Mol Cell Biol Date: 2007-04-16 Impact factor: 4.272

2. Activation and overexpression of centrosome kinase BTAK/Aurora-A in human ovarian cancer.

Authors: Tatiana M Gritsko; Domenico Coppola; June E Paciga; Lin Yang; Mei Sun; Sue A Shelley; James V Fiorica; Santo V Nicosia; Jin Q Cheng
Journal: Clin Cancer Res Date: 2003-04 Impact factor: 12.531

3. Association of centrosomal kinase STK15/BTAK mRNA expression with chromosomal instability in human breast cancers.

Authors: Y Miyoshi; K Iwao; C Egawa; S Noguchi
Journal: Int J Cancer Date: 2001-05-01 Impact factor: 7.396

4. Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1.

Authors: M Kimura; S Kotani; T Hattori; N Sumi; T Yoshioka; K Todokoro; Y Okano
Journal: J Biol Chem Date: 1997-05-23 Impact factor: 5.157

5. Genetic aberrations detected by comparative genomic hybridization predict outcome in node-negative breast cancer.

Authors: J J Isola; O P Kallioniemi; L W Chu; S A Fuqua; S G Hilsenbeck; C K Osborne; F M Waldman
Journal: Am J Pathol Date: 1995-10 Impact factor: 4.307

6. Roles of aurora-A kinase in mitotic entry and G2 checkpoint in mammalian cells.

Authors: Tomotoshi Marumoto; Toru Hirota; Tetsuro Morisaki; Naoko Kunitoku; Dongwei Zhang; Yasuko Ichikawa; Takashi Sasayama; Shinji Kuninaka; Tatsuyuki Mimori; Norihiko Tamaki; Masashi Kimura; Yukio Okano; Hideyuki Saya
Journal: Genes Cells Date: 2002-11 Impact factor: 1.891

7. Tumour-amplified kinase BTAK is amplified and overexpressed in gastric cancers with possible involvement in aneuploid formation.

Authors: C Sakakura; A Hagiwara; R Yasuoka; Y Fujita; M Nakanishi; K Masuda; K Shimomura; Y Nakamura; J Inazawa; T Abe; H Yamagishi
Journal: Br J Cancer Date: 2001-03-23 Impact factor: 7.640

8. Centrosomal kinases, HsAIRK1 and HsAIRK3, are overexpressed in primary colorectal cancers.

Authors: T Takahashi; M Futamura; N Yoshimi; J Sano; M Katada; Y Takagi; M Kimura; T Yoshioka; Y Okano; S Saji
Journal: Jpn J Cancer Res Date: 2000-10

9. VX-680, a potent and selective small-molecule inhibitor of the Aurora kinases, suppresses tumor growth in vivo.

Authors: Elizabeth A Harrington; David Bebbington; Jeff Moore; Richele K Rasmussen; Abi O Ajose-Adeogun; Tomoko Nakayama; Joanne A Graham; Cecile Demur; Thierry Hercend; Anita Diu-Hercend; Michael Su; Julian M C Golec; Karen M Miller
Journal: Nat Med Date: 2004-02-22 Impact factor: 53.440