Literature DB >> 18701664

Whole blood as an alternative to plasma for detection of hepatitis C virus RNA.

Hubert Darius J Daniel1, Joel David, Paul R Grant, Jeremy A Garson, George M Chandy, Priya Abraham.   

Abstract

Peripheral blood mononuclear cells are reported to be one of the extrahepatic replication sites contributing to the persistence of hepatitis C virus (HCV) infection. Whole-blood and plasma samples from 61 individuals were compared as sources for the detection of HCV RNA. Forty-four of the individuals were receiving antiviral therapy, while 17 were treatment naïve. The quantitation of HCV RNA was done by a sensitive in-house real-time reverse transcription-PCR. When the viral loads in the two types of samples were compared, a correlation coefficient of 0.858 (P < 0.001) was found, indicating that plasma and whole blood are equally acceptable sources for testing for HCV RNA.

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Year:  2008        PMID： 18701664      PMCID： PMC2576615          DOI： 10.1128/JCM.00045-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

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2.  Distribution of hepatitis C virus (HCV) RNA in whole blood and blood cell fractions: plasma HCV RNA analysis underestimates circulating virus load.

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Journal:  J Infect Dis       Date:  1997-07       Impact factor: 5.226

3.  Surreptitious hepatitis C virus (HCV) infection detected in the majority of patients with cryptogenic chronic hepatitis and negative HCV antibody tests.

Authors:  W N Schmidt; P Wu; J Cederna; F A Mitros; D R LaBrecque; J T Stapleton
Journal:  J Infect Dis       Date:  1997-07       Impact factor: 5.226

4.  The prevalence of hepatitis C virus infection in the United States, 1988 through 1994.

Authors:  M J Alter; D Kruszon-Moran; O V Nainan; G M McQuillan; F Gao; L A Moyer; R A Kaslow; H S Margolis
Journal:  N Engl J Med       Date:  1999-08-19       Impact factor: 91.245

5.  Rapid isolation of HCV RNA from Catrimox-lysed whole blood using QIAamp spin columns.

Authors:  S A Ali; B Kubik; H Gulle; M M Eibl; A Steinkasserer
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6.  Hepatitis C: global prevalence.

Authors: 
Journal:  Wkly Epidemiol Rec       Date:  1997-11-14

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Authors:  L Cook; A M Ross; G B Knight; V Agnello
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

8.  Hepatitis C virus may infect extrahepatic tissues in patients with hepatitis C.

Authors:  Fu-Ming Yan; An-Shen Chen; Fei Hao; Xi-Ping Zhao; Chang-Hai Gu; Li-Bin Zhao; Dong-Liang Yang; Lian-Jie Hao
Journal:  World J Gastroenterol       Date:  2000-12       Impact factor: 5.742

9.  The clinical utility of using Catrimox-14-treated whole blood in detecting hepatitis C virus RNA.

Authors:  Keyur Patel; Jeff Albrecht; Erin Owens; Anouk Dev; Shanon Heaton; Stephen Pianko; Paul J Pockros; Andrew Conrad; Lawrence M Blatt; John G McHutchison
Journal:  Antivir Ther       Date:  2005

10.  Effect of interferon therapy on hepatitis C virus RNA in whole blood, plasma, and peripheral blood mononuclear cells.

Authors:  W N Schmidt; P Wu; D Brashear; D Klinzman; M J Phillips; D R LaBrecque; J T Stapleton
Journal:  Hepatology       Date:  1998-10       Impact factor: 17.425
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  2 in total

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Authors:  Tony Bruns; Katrin Steinmetzer; Eugen Ermantraut; Andreas Stallmach
Journal:  J Clin Microbiol       Date:  2009-08-19       Impact factor: 5.948

2.  Development of an In-House TaqMan Real Time RT-PCR Assay to Quantify Hepatitis C Virus RNA in Serum and Peripheral Blood Mononuclear Cells in Patients With Chronic Hepatitis C Virus Infection.

Authors:  Bahman Khalvati Fahlyani; Abbas Behzad-Behbahani; Seiied Alireza Taghavi; Ali Farhadi; Saeede Salehi; Setare Adibzadeh; Farzaneh Aboualizadeh; Parniyan Alavi; Negin Nikouyan; Mohammad Ali Okhovat; Reza Ranjbaran; Gholam Reza Rafiei Dehbidi; Arash Shakibzadeh
Journal:  Hepat Mon       Date:  2015-08-31       Impact factor: 0.660
  2 in total

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