The 14 bp deletion polymorphisms in HLA-G gene play an important role in the expression of soluble HLA-G in plasma.

Soluble human leukocyte antigen-G (sHLA-G) functions as a multiple immunoregulator. A 14 bp insertion (+14 bp)/deletion (-14 bp) polymorphism in exon 8 of the HLA-G gene has been proposed to be associated with HLA-G mRNA stability and the expression of HLA-G. In the current study, a total of 150 normal Chinese Han population had been genotyped for the +14 bp/-14 bp polymorphism, and the expression of plasma sHLA-G was determined with enzyme-linked immunosorbent assay in these case-matched plasma. Data showed that genotype of 14 bp polymorphism was significantly associated with sHLA-G expression. Plasma sHLA-G level with the +14 bp/+14 bp genotype was dramatically lower than that with +14 bp/-14 bp (P = 0.004) and -14 bp/-14 bp genotypes (P = 0.003), while no dramatic difference was observed between the +14 bp/-14 bp and -14 bp/-14 bp genotypes (P > 0.05). In both males and females, plasma sHLA-G with the +14 bp/+14 bp genotype was also significantly lower when compared with other two respective 14 bp genotypes. Data also showed that sHLA-G expression was unrelated to gender. This study suggests that the 14 bp deletion polymorphism in the HLA-G gene plays an important role in sHLA-G expression and that interpretation of the potential biological functions of sHLA-G should be made with caution, taking the polymorphism into consideration.