AIM: To examine the effects of various root canal irrigants on cervical dentine permeability by monitoring the diffusion of hydrogen peroxide (H(2)O(2)). METHODOLOGY:Sixty cervical dentine discs were prepared from human third molar teeth. After removal of enamel and cementum, the outer dentine surface was etched with 17% ethylenediamine tetraacetic acid (EDTA) for 1 min. The dentine discs were randomly assigned to five groups according to the irrigant used: A, saline solution; B, 2.5% sodium hypochlorite solution (NaOCl); C, 5% NaOCl; D, 17% EDTA and 2.5% NaOCl; E, 17% EDTA and 5% NaOCl. After irrigation on the inner dentine surface with the various solutions, the diffusion of 30% H(2)O(2) was evaluated through each disc using a plastic-split chamber. H(2)O(2) was applied to the inner-side chamber, while the outer-side chamber was filled with de-ionised water. After 30 min of application of H(2)O(2), the solution in the outer-side chamber was collected to determine the concentration of H(2)O(2) using a spectrophotometer. RESULTS: The penetration of H(2)O(2) through dentine in group E was significantly highest, followed by groups D, C, B and A respectively (one-way anova, P < 0.05). CONCLUSIONS: Among the irrigants used, 17% EDTA and 5% NaOCl had the greatest effect in increasing dentinal permeability to H(2)O(2).
RCT Entities:
AIM: To examine the effects of various root canal irrigants on cervical dentine permeability by monitoring the diffusion of hydrogen peroxide (H(2)O(2)). METHODOLOGY: Sixty cervical dentine discs were prepared from human third molar teeth. After removal of enamel and cementum, the outer dentine surface was etched with 17% ethylenediamine tetraacetic acid (EDTA) for 1 min. The dentine discs were randomly assigned to five groups according to the irrigant used: A, saline solution; B, 2.5% sodium hypochlorite solution (NaOCl); C, 5% NaOCl; D, 17% EDTA and 2.5% NaOCl; E, 17% EDTA and 5% NaOCl. After irrigation on the inner dentine surface with the various solutions, the diffusion of 30% H(2)O(2) was evaluated through each disc using a plastic-split chamber. H(2)O(2) was applied to the inner-side chamber, while the outer-side chamber was filled with de-ionised water. After 30 min of application of H(2)O(2), the solution in the outer-side chamber was collected to determine the concentration of H(2)O(2) using a spectrophotometer. RESULTS: The penetration of H(2)O(2) through dentine in group E was significantly highest, followed by groups D, C, B and A respectively (one-way anova, P < 0.05). CONCLUSIONS: Among the irrigants used, 17% EDTA and 5% NaOCl had the greatest effect in increasing dentinal permeability to H(2)O(2).
Authors: Janir Alves Soares; Maria Auxiliadora Roque de Carvalho; Suelleng Maria Cunha Santos Soares; Rodrigo Dantas Pereira; Manoel Brito-Júnior; Paula Prazeres Magalhães; Isabel Alessandra Miranda Nunes; Rudys Rodolpho de Jesus Tavarez; Claudia de Castro Rizzi; Rafaela Nogueira Moreira; Luiz de Macêdo Farias; Etevaldo Matos Maia Filho Journal: ScientificWorldJournal Date: 2019-01-01