Literature DB >> 18695964

EasyBeacons for the detection of methylation status of single CpG duplets.

Ulf B Christensen1.   

Abstract

This chapter describes two different fast protocols for optimizing EasyBeacons for the challenging detection of the methylation status of a single CpG duplet in bisulfitetreated DNA. EasyBeacons can be used in multiplex detections even if they do not have the same affinity for their respective targets giving very specific results for both realtime polymerase chain reaction (real-time PCR) detection and endpoint analysis using inexpensive master mixes. The technology described in this chapter is a very competitive alternative to other real-time PCR technologies.

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Year:  2008        PMID： 18695964     DOI： 10.1007/978-1-60327-040-3_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  KRAS genotyping by digital PCR combined with melting curve analysis.

Authors:  Junko Tanaka; Tatsuo Nakagawa; Akiko Shiratori; Yuzuru Shimazaki; Chihiro Uematsu; Masao Kamahori; Takahide Yokoi; Kunio Harada; Yoshinobu Kohara
Journal:  Sci Rep       Date:  2019-02-22       Impact factor: 4.379

2.  Association of serum surfactant protein D and SFTPD gene variants with asthma in Danish children, adolescents, and young adults.

Authors:  Benjamin Hoffmann-Petersen; Raymond Suffolk; Jens J H Petersen; Thomas H Petersen; Charlotte Brasch-Andersen; Arne Høst; Susanne Halken; Grith L Sorensen; Lone Agertoft
Journal:  Immun Inflamm Dis       Date:  2021-11-15

3.  Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants.

Authors:  Sebastian Juul; Malene Roed Spiegelhauer; Mette Neve Petersen; Katharina Kirkegaard Flugt; Nikolaj Vestergaard Hansen; Helene Larsen; Per Bo Jensen; Ulf Bech Christensen; Rasmus Koefoed Petersen; Lennart Friis-Hansen
Journal:  Sci Rep       Date:  2022-07-29       Impact factor: 4.996

4.  Biomarkers in asthma in the context of atopic dermatitis in young children.

Authors:  Millie Nguyen Basu; Charlotte G Mortz; Tina Kold Jensen; Torben Barington; Kate Lykke Lambertsen; Susanne Halken
Journal:  Pediatr Allergy Immunol       Date:  2022-07       Impact factor: 5.464
  4 in total

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