Literature DB >> 1869446

A method for enzyme- and immunohistochemical staining of large frozen specimens.

B Holmbom1, M Lindström, U Näslund, L E Thornell.   

Abstract

A method for large specimen cryosectioning is described. Specimens of pig heart ventricles were lightly fixed by microwave irradiation, embedded in 10% gelatin, frozen in hexan chilled with dry ice, and sectioned using an LKB 2250 PMW cryomicrotome. The sections were collected on transparent film and transferred onto glass slides. Standard histological, enzyme- and immuno-histochemical staining techniques were used. The present method allowed cryosectioning with sections of good quality which could be used for enzyme- and immuno-staining. For studies of experimental myocardial infarction, staining for phosphorylase and Periodic Acid Schiff outlined the ischemic area and antibodies against plasma fibronectin and fibrinogen delineated the infarcted myocardium.

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Year:  1991        PMID： 1869446     DOI： 10.1007/bf00315739

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  24 in total

1.  The specificity of the histochemical method for adenosine triphosphatase.

Authors:  H A PADYKULA; E HERMAN
Journal:  J Histochem Cytochem       Date:  1955-05       Impact factor: 2.479

2.  Peritubular myoid cells of human and rat testis are smooth muscle cells that contain desmin-type intermediate filaments.

Authors:  I Virtanen; M Kallajoki; O Närvänen; J Paranko; L E Thornell; M Miettinen; V P Lehto
Journal:  Anat Rec       Date:  1986-05

3.  Application of cryoultramicrotomy to immunocytochemistry.

Authors:  K T Tokuyasu
Journal:  J Microsc       Date:  1986-08       Impact factor: 1.758

Review 4.  Infarct size--can it be measured or modified in humans?

Authors:  L M Buja; J T Willerson
Journal:  Prog Cardiovasc Dis       Date:  1987 Jan-Feb       Impact factor: 8.194

5.  Distribution of different fibre types in human skeletal muscles. I. Method for the preparation and analysis of cross-sections of whole tibialis anterior.

Authors:  K B Henriksson-Larsén; J Lexell; M Sjöström
Journal:  Histochem J       Date:  1983-02

6.  Distribution of different fibre types in human skeletal muscles. 2. A study of cross-sections of whole m. vastus lateralis.

Authors:  J Lexell; K Henriksson-Larsén; M Sjöström
Journal:  Acta Physiol Scand       Date:  1983-01

7.  Early loss of myocardial myoglobin detected immunohistochemically following occlusion of the coronary artery in rats.

Authors:  K Nomoto; N Mori; T Shoji; K Nakamura
Journal:  Exp Mol Pathol       Date:  1987-12       Impact factor: 3.362

8.  Microwave stabilization versus chemical fixation. A morphometric study in glycolmethacrylate- and paraffin-embedded tissue.

Authors:  H J Van de Kant; D G De Rooij; M E Boon
Journal:  Histochem J       Date:  1990 Jun-Jul

9.  Limitation of myocardial infarct size by superoxide dismutase as an adjunct to reperfusion after different durations of coronary occlusion in the pig.

Authors:  U Näslund; S Häggmark; G Johansson; S L Marklund; S Reiz
Journal:  Circ Res       Date:  1990-05       Impact factor: 17.367

10.  Microwave fixation: its potential for routine techniques, histochemistry, immunocytochemistry and electron microscopy.

Authors:  D Hopwood; G Coghill; J Ramsay; G Milne; M Kerr
Journal:  Histochem J       Date:  1984-11
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  3 in total

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Authors:  B Holmbom; U Näslund; A Eriksson; I Virtanen; L E Thornell
Journal:  Histochemistry       Date:  1993-04

2.  Distribution of prolyl oligopeptidase in the mouse whole-body sections and peripheral tissues.

Authors:  Timo T Myöhänen; Jarkko I Venäläinen; J Arturo García-Horsman; Marjo Piltonen; Pekka T Männistö
Journal:  Histochem Cell Biol       Date:  2008-07-11       Impact factor: 4.304

3.  Re-evaluation of sarcolemma injury and muscle swelling in human skeletal muscles after eccentric exercise.

Authors:  Ji-Guo Yu; Jing-Xia Liu; Lena Carlsson; Lars-Eric Thornell; Per S Stål
Journal:  PLoS One       Date:  2013-04-15       Impact factor: 3.240
  3 in total

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