Literature DB >> 18691542

A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis.

Chun Yi Liau1, Chung-Sheng Lin.   

Abstract

A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated that our method is more sensitive and less complicated than the conventional Calcofluor white M2R staining.

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Year:  2008        PMID： 18691542     DOI： 10.1263/jbb.106.111

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  7 in total

1.  Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica.

Authors:  Jeong-Nam Park; Chang Pyo Han; Dong-Jik Lee; Seon Ah Cheon; Hyun Ah Kang
Journal:  J Microbiol       Date:  2014-03-29       Impact factor: 3.422

2.  The occurrence of chitin in the hemocytes of invertebrates.

Authors:  Elizabeth A C Heath-Heckman; Margaret J McFall-Ngai
Journal:  Zoology (Jena)       Date:  2011-07-01       Impact factor: 2.240

3.  Antifungal performance of extracellular chitinases and culture supernatants of Streptomyces galilaeus CFFSUR-B12 against Mycosphaerella fijiensis Morelet.

Authors:  Benjamín Moreno Castillo; Michael F Dunn; Karina Guillén Navarro; Francisco Holguín Meléndez; Magdalena Hernández Ortiz; Sergio Encarnación Guevara; Graciela Huerta Palacios
Journal:  World J Microbiol Biotechnol       Date:  2016-02-12       Impact factor: 3.312

4.  Construction and characterization of a thermostable whole-cell chitinolytic enzyme using yeast surface display.

Authors:  Xiaobo Li; Xiaobao Jin; Xuemei Lu; Fujiang Chu; Juan Shen; Yan Ma; Manyu Liu; Jiayong Zhu
Journal:  World J Microbiol Biotechnol       Date:  2014-06-07       Impact factor: 3.312

5.  The chitinolytic activities of Streptomyces sp. TH-11.

Authors:  Kim-Chi Hoang; Tzu-Hsuan Lai; Chung-Sheng Lin; Ying-Tsong Chen; Chun-Yi Liau
Journal:  Int J Mol Sci       Date:  2010-12-27       Impact factor: 5.923

6.  Directional transfer of a multiple-allele male sterile line in Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee.

Authors:  Qiu Shi Wang; Xi Zhang; Cheng Yu Li; Zhi Yong Liu; Hui Feng
Journal:  Breed Sci       Date:  2014-06-01       Impact factor: 2.086

7.  Purification and characterization of an extracellular thermo-alkali stable, metal tolerant chitinase from Streptomyces chilikensis RC1830 isolated from a brackish water lake sediment.

Authors:  Lopamudra Ray; Ananta Narayan Panda; Samir Ranjan Mishra; Ajit Kumar Pattanaik; Tapan Kumar Adhya; Mrutyunjay Suar; Vishakha Raina
Journal:  Biotechnol Rep (Amst)       Date:  2019-01-29
  7 in total

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