Literature DB >> 18689475

Role of the N-terminal domain of phiC31 integrase in attB-attP synapsis.

Paul A Rowley1, Margaret C M Smith.   

Abstract

PhiC31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to synapse. We show here that substitutions in amino acid V129 in the NTD can lead to defects in synapsis and DNA cleavage, indicating that the NTD also has an important role in synapsis.

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Year:  2008        PMID： 18689475      PMCID： PMC2566186          DOI： 10.1128/JB.00612-08

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  Application of multiple sequence alignment profiles to improve protein secondary structure prediction.

Authors:  J A Cuff; G J Barton
Journal:  Proteins       Date:  2000-08-15

Review 2.  Diversity in the serine recombinases.

Authors:  Margaret C M Smith; Helena M Thorpe
Journal:  Mol Microbiol       Date:  2002-04       Impact factor: 3.501

3.  A model for the gamma delta resolvase synaptic complex.

Authors:  G J Sarkis; L L Murley; A E Leschziner; M R Boocock; W M Stark; N D Grindley
Journal:  Mol Cell       Date:  2001-09       Impact factor: 17.970

4.  The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB.

Authors:  Pallavi Ghosh; Amy I Kim; Graham F Hatfull
Journal:  Mol Cell       Date:  2003-11       Impact factor: 17.970

5.  Synapsis and DNA cleavage in phiC31 integrase-mediated site-specific recombination.

Authors:  Matthew C A Smith; Rob Till; Kevin Brady; Panos Soultanas; Helena Thorpe; Margaret C M Smith
Journal:  Nucleic Acids Res       Date:  2004-05-11       Impact factor: 16.971

6.  In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family.

Authors:  H M Thorpe; M C Smith
Journal:  Proc Natl Acad Sci U S A       Date:  1998-05-12       Impact factor: 11.205

7.  Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site.

Authors:  W Yang; T A Steitz
Journal:  Cell       Date:  1995-07-28       Impact factor: 41.582

8.  Mutants of Tn3 resolvase which do not require accessory binding sites for recombination activity.

Authors:  P H Arnold; D G Blake; N D Grindley; M R Boocock; W M Stark
Journal:  EMBO J       Date:  1999-03-01       Impact factor: 11.598

9.  Mycobacteriophage Bxb1 integrates into the Mycobacterium smegmatis groEL1 gene.

Authors:  Amy I Kim; Pallavi Ghosh; Michelle A Aaron; Lori A Bibb; Shruti Jain; Graham F Hatfull
Journal:  Mol Microbiol       Date:  2003-10       Impact factor: 3.501

10.  A motif in the C-terminal domain of phiC31 integrase controls the directionality of recombination.

Authors:  Paul A Rowley; Matthew C A Smith; Ellen Younger; Margaret C M Smith
Journal:  Nucleic Acids Res       Date:  2008-05-23       Impact factor: 16.971
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  9 in total

Review 1.  Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).

Authors:  Richard H Baltz
Journal:  J Ind Microbiol Biotechnol       Date:  2011-12-13       Impact factor: 3.346

Review 2.  The ins and outs of serine integrase site-specific recombination.

Authors:  Karen Rutherford; Gregory D Van Duyne
Journal:  Curr Opin Struct Biol       Date:  2014-02-11       Impact factor: 6.809

3.  Intrasubunit and intersubunit interactions controlling assembly of active synaptic complexes during Hin-catalyzed DNA recombination.

Authors:  John K Heiss; Erin R Sanders; Reid C Johnson
Journal:  J Mol Biol       Date:  2011-06-25       Impact factor: 5.469

4.  Coiled-coil interactions mediate serine integrase directionality.

Authors:  Kushol Gupta; Robert Sharp; Jimmy B Yuan; Huiguang Li; Gregory D Van Duyne
Journal:  Nucleic Acids Res       Date:  2017-07-07       Impact factor: 16.971

5.  Mutational analysis of highly conserved residues in the phage phiC31 integrase reveals key amino acids necessary for the DNA recombination.

Authors:  Shaohui Liu; Jinfang Ma; Wei Wang; Maoxiang Zhang; Qingting Xin; Siman Peng; Rongxiu Li; Huanzhang Zhu
Journal:  PLoS One       Date:  2010-01-25       Impact factor: 3.240

Review 6.  Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences.

Authors:  Helen Atkinson; Ronald Chalmers
Journal:  Genetica       Date:  2010-01-19       Impact factor: 1.633

7.  Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis.

Authors:  Kimihiro Abe; Takuo Takamatsu; Tsutomu Sato
Journal:  Nucleic Acids Res       Date:  2017-06-20       Impact factor: 16.971

8.  DNA binding and synapsis by the large C-terminal domain of phiC31 integrase.

Authors:  Andrew R McEwan; Paul A Rowley; Margaret C M Smith
Journal:  Nucleic Acids Res       Date:  2009-06-10       Impact factor: 16.971

9.  Control of the Serine Integrase Reaction: Roles of the Coiled-Coil and Helix E Regions in DNA Site Synapsis and Recombination.

Authors:  Sridhar Mandali; Reid C Johnson
Journal:  J Bacteriol       Date:  2021-07-22       Impact factor: 3.490
  9 in total

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