Literature DB >> 18688713

Immunocytochemical assessment of p53 protein to detect malignancy in increased cell-yield brush cytology from the biliopancreatic tree.

Vincenzo Villanacci1, Renzo Cestari, Stefano Giulini, Paolo Cengia, Guido Missale, Angiola Berenzi, Elisa Rossi, Massimo Bonardi, Luca Baiocchi, Gabrio Bassotti.   

Abstract

BACKGROUND: Malignancies arising from the biliopancreatic tree are often diagnostic challenges for the gastroenterologist and the pathologist, especially when strictures without masses are present. AIM: To evaluate the diagnostic yield of p53 immunocytology for the detection of malignancies in material obtained by biliopancreatic tree brushing by means of an increased cell-yield procedure. PATIENTS AND METHODS: Cytologic specimens obtained from biliary and pancreatic tree brushing in 24 patients with biliary strictures suspected for malignancy were assessed by conventional Papanicolau staining and p53 immunocytochemistry.
RESULTS: Papanicolau staining detected 67% and p53 87% of the malignancies in the study group. p53 immunocytology displayed excellent sensitivity, specificity, and diagnostic accuracy.
CONCLUSIONS: p53 immunocytology may represent a useful diagnostic tool in the detection of malignancies from biliary and pancreatic tree brushing, especially when using an increasing cell-yield procedure.

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Year:  2008        PMID: 18688713     DOI: 10.1007/s10620-008-0431-7

Source DB:  PubMed          Journal:  Dig Dis Sci        ISSN: 0163-2116            Impact factor:   3.199


  18 in total

1.  Value of p53 immunostaining in pancreatico-biliary brush cytology specimens.

Authors:  C J Stewart; G M Burke
Journal:  Diagn Cytopathol       Date:  2000-11       Impact factor: 1.582

2.  The combination of stricture dilation, endoscopic needle aspiration, and biliary brushings significantly improves diagnostic yield from malignant bile duct strictures.

Authors:  R J Farrell; A K Jain; S L Brandwein; H Wang; R Chuttani; D K Pleskow
Journal:  Gastrointest Endosc       Date:  2001-11       Impact factor: 9.427

Review 3.  The p53 tumour suppressor gene and product.

Authors:  A J Levine
Journal:  Cancer Surv       Date:  1992

Review 4.  p53: tumor suppression through control of the cell cycle.

Authors:  S E Kern
Journal:  Gastroenterology       Date:  1994-06       Impact factor: 22.682

5.  Diagnostic value of brush cytology in the diagnosis of bile duct carcinoma: a study in 65 patients with bile duct strictures.

Authors:  M Rabinovitz; A B Zajko; T Hassanein; B Shetty; K M Bron; R R Schade; J S Gavaler; G Block; D H Van Thiel; A Dekker
Journal:  Hepatology       Date:  1990-10       Impact factor: 17.425

6.  [Malignant biliary papillomatosis: analysis of p53 expression].

Authors:  Mostafa Ahaouche; Dominique Cazals-Hatem; Thierry Watrin; Gilles Arcangeli; Philippe Ruszniewski; Claude Degott
Journal:  Ann Pathol       Date:  2004-09       Impact factor: 0.407

7.  Molecular diagnostic testing as an adjunct to morphologic evaluation of pancreatic ductal system brushings: potential augmentation for diagnostic sensitivity.

Authors:  Carlynn Willmore-Payne; Keith E Volmar; Michael A Huening; Joseph A Holden; Lester J Layfield
Journal:  Diagn Cytopathol       Date:  2007-04       Impact factor: 1.582

Review 8.  Evaluation and management of periampullary tumors.

Authors:  William A Ross; Mike M Bismar
Journal:  Curr Gastroenterol Rep       Date:  2004-10

9.  An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms.

Authors:  I O Baas; J W Mulder; G J Offerhaus; B Vogelstein; S R Hamilton
Journal:  J Pathol       Date:  1994-01       Impact factor: 7.996

Review 10.  Ampullary adenoma: clinical manifestations, diagnosis, and treatment.

Authors:  John A Martin; Gregory B Haber
Journal:  Gastrointest Endosc Clin N Am       Date:  2003-10
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  1 in total

1.  Effects of endoscopic sphincterotomy on biliary epithelium: a case-control study.

Authors:  John Kalaitzis; Antonios Vezakis; George Fragulidis; Irene Anagnostopoulou; Spyros Rizos; Efstathios Papalambros; Andreas Polydorou
Journal:  World J Gastroenterol       Date:  2012-02-28       Impact factor: 5.742

  1 in total

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