Literature DB >> 1868856

GDP-fucose: beta-N-acetylglucosamine (Fuc to (Fuc alpha 1----6GlcNAc)-Asn-peptide)alpha 1----3-fucosyltransferase activity in honeybee (Apis mellifica) venom glands. The difucosylation of asparagine-bound N-acetylglucosamine.

E Staudacher1, F Altmann, J Glössl, L März, H Schachter, J P Kamerling, K Hård, J F Vliegenthart.   

Abstract

Incubation of honeybee (Apis mellifica) venom-gland extracts with GDP-[14C]fucose and GlcNAc beta 1----2Man alpha 1----6(GlcNAc beta 1----2Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4(Fuc alpha 1----6)GlcNAc beta 1----N-Asn-peptide(NAc) gave a labeled product in 40% yield. Analysis by 500-MHz 1H-NMR spectroscopy indicated the transferred fucose-(Fuc) residue to be alpha 1----3-linked to the Asn-bound GlcNAc. Further proof was provided by one-dimensional and two-dimensional 1H-NMR analysis of the incubation mixture, after incubation with beta-N-acetylhexosaminidase. The established carbohydrate structure (formula; see text) proves the existence of a novel alpha 1----3-fucosyltransferase with the ability to effect difucosylation of the Asn-bound GlcNAc in N-glycans.

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Year:  1991        PMID： 1868856     DOI： 10.1111/j.1432-1033.1991.tb16179.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  12 in total

1.  A novel approach for identification and characterization of glycoproteins using a hybrid linear ion trap/FT-ICR mass spectrometer.

Authors:  Scott M Peterman; Joseph J Mulholland
Journal:  J Am Soc Mass Spectrom       Date:  2006-01-10       Impact factor: 3.109

Review 2.  Revealing the anti-HRP epitope in Drosophila and Caenorhabditis.

Authors:  Katharina Paschinger; Dubravko Rendić; Iain B H Wilson
Journal:  Glycoconj J       Date:  2008-08-26       Impact factor: 2.916

Review 3.  Insect cells as hosts for the expression of recombinant glycoproteins.

Authors:  F Altmann; E Staudacher; I B Wilson; L März
Journal:  Glycoconj J       Date:  1999-02       Impact factor: 2.916

4.  Towards abolition of immunogenic structures in insect cells: characterization of a honey-bee (Apis mellifera) multi-gene family reveals both an allergy-related core alpha1,3-fucosyltransferase and the first insect Lewis-histo-blood-group-related antigen-synthesizing enzyme.

Authors:  Dubravko Rendić; Jaroslav Klaudiny; Ute Stemmer; Julia Schmidt; Katharina Paschinger; Iain B H Wilson
Journal:  Biochem J       Date:  2007-02-15       Impact factor: 3.857

5.  Functional purification and characterization of a GDP-fucose: beta-N-acetylglucosamine (Fuc to Asn linked GlcNAc) alpha 1,3-fucosyltransferase from mung beans.

Authors:  E Staudacher; T Dalik; P Wawra; F Altmann; L März
Journal:  Glycoconj J       Date:  1995-12       Impact factor: 2.916

Review 6.  The joys of HexNAc. The synthesis and function of N- and O-glycan branches.

Authors:  H Schachter
Journal:  Glycoconj J       Date:  2000 Jul-Sep       Impact factor: 2.916

7.  Structural characterization of the N-linked oligosaccharides derived from HIVgp120 expressed in lepidopteran cells.

Authors:  T D Butters; B Yudkin; G S Jacob; I M Jones
Journal:  Glycoconj J       Date:  1998-01       Impact factor: 2.916

8.  Bacterial nodulation protein NodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin.

Authors:  C Quinto; A H Wijfjes; G V Bloemberg; L Blok-Tip; I M López-Lara; B J Lugtenberg; J E Thomas-Oates; H P Spaink
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-29       Impact factor: 11.205

9.  Strict order of (Fuc to Asn-linked GlcNAc) fucosyltransferases forming core-difucosylated structures.

Authors:  E Staudacher; L März
Journal:  Glycoconj J       Date:  1998-04       Impact factor: 2.916

10.  Alpha 1-6(alpha 1-3)-difucosylation of the asparagine-bound N-acetylglucosamine in honeybee venom phospholipase A2.

Authors:  E Staudacher; F Altmann; L März; K Hård; J P Kamerling; J F Vliegenthart
Journal:  Glycoconj J       Date:  1992-04       Impact factor: 2.916
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