BACKGROUND: The FOXP3 mRNA expression and the other regulatory T cell-related molecules were investigated and compared with clinicopathological parameters in human primary breast cancer. METHOD: This study included 136 breast cancer patients operated in our department from 2003 to 2006. Total RNA was extracted from frozen normal breast and breast cancer tissues, and the expression of FOXP3, IL-10, TGFbeta1 and CCL22 mRNA was evaluated using quantitative real-time RT-PCR. RESULT: FOXP3, IL-10, TGFbeta1 and CCL22 mRNA expressions were significantly higher in cancer tissue than in normal tissue, not only at pT1, 2, and 3 stages but also at the DCIS stage. There were positive correlations between FOXP3 and IL-10, FOXP3 and TGFbeta1, as well as FOXP3 and CCL22 mRNA expressions, respectively. FOXP3 and IL-10 mRNA expressions were significantly upregulated in PgR-negative or HER2-positive tumors. CONCLUSION: These results suggest that regulatory T cells are involved in tumor onset and progression in human primary breast cancer, possibly contributing to poor prognosis of patients with breast cancer.
BACKGROUND: The FOXP3 mRNA expression and the other regulatory T cell-related molecules were investigated and compared with clinicopathological parameters in human primary breast cancer. METHOD: This study included 136 breast cancerpatients operated in our department from 2003 to 2006. Total RNA was extracted from frozen normal breast and breast cancer tissues, and the expression of FOXP3, IL-10, TGFbeta1 and CCL22 mRNA was evaluated using quantitative real-time RT-PCR. RESULT: FOXP3, IL-10, TGFbeta1 and CCL22 mRNA expressions were significantly higher in cancer tissue than in normal tissue, not only at pT1, 2, and 3 stages but also at the DCIS stage. There were positive correlations between FOXP3 and IL-10, FOXP3 and TGFbeta1, as well as FOXP3 and CCL22 mRNA expressions, respectively. FOXP3 and IL-10 mRNA expressions were significantly upregulated in PgR-negative or HER2-positive tumors. CONCLUSION: These results suggest that regulatory T cells are involved in tumor onset and progression in human primary breast cancer, possibly contributing to poor prognosis of patients with breast cancer.
Authors: Jaime Fornetti; Holly A Martinson; Courtney B Betts; Traci R Lyons; Sonali Jindal; Qiuchen Guo; Lisa M Coussens; Virginia F Borges; Pepper Schedin Journal: J Mammary Gland Biol Neoplasia Date: 2014-06-22 Impact factor: 2.673
Authors: Wei Tan; Weizhou Zhang; Amy Strasner; Sergei Grivennikov; Jin Q Cheng; Robert M Hoffman; Michael Karin Journal: Nature Date: 2011-02-16 Impact factor: 49.962
Authors: Nina C Dempsey; Francesca Leoni; H Elyse Ireland; Christine Hoyle; John H H Williams Journal: J Leukoc Biol Date: 2009-12-10 Impact factor: 4.962