Unusual organization of a ribosomal protein operon in the plastid genome of Cryptomonas phi: evolutionary considerations.

The region of the plastid genome containing the genes for ribosomal proteins S12 and S7 and the elongation factor Tu (corresponding to three of the four str operon genes of Escherichia coli) was investigated in the unicellular marine alga Cryptomonas. Sequence analysis shows the gene organization to be rps12-60 bp spacer-rps7-68 bp spacer-tufA. No introns are present in any of the genes. Comparisons of the deduced amino acid sequence of these genes with homologues from other organisms show rps12 to be very highly conserved, except at the amino terminus, and rps7 and tufA to be less well-conserved. Transcript analysis suggests that these genes are co-transcribed along with several up and/or down-stream genes. The evolutionary significance of this unique gene organization is discussed.