Literature DB >> 18684625

Synthesis and characterization of BODIPY-labeled colchicine.

Leggy A Arnold1, Patricia Ranaivo, R Kiplin Guy.   

Abstract

Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells.

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Year:  2008        PMID： 18684625      PMCID： PMC2631555          DOI： 10.1016/j.bmcl.2008.07.068

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  19 in total

1.  Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain.

Authors:  Raimond B G Ravelli; Benoît Gigant; Patrick A Curmi; Isabelle Jourdain; Sylvie Lachkar; André Sobel; Marcel Knossow
Journal:  Nature       Date:  2004-03-11       Impact factor: 49.962

2.  Microtubule assembly in the absence of added nucleotides.

Authors:  M L Shelanski; F Gaskin; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

3.  The colchicine-binding protein of mammalian brain and its relation to microtubules.

Authors:  R C Weisenberg; G G Borisy; E W Taylor
Journal:  Biochemistry       Date:  1968-12       Impact factor: 3.162

4.  In vitro reconstitution of calf brain microtubules: effects of solution variables.

Authors:  J C Lee; S N Timasheff
Journal:  Biochemistry       Date:  1977-04-19       Impact factor: 3.162

5.  Fluorescent deacetylcolchicine. New aspects of its activity and localization in PtK-1 cells.

Authors:  E Moll; B Manz; S Mocikat; H P Zimmermann
Journal:  Exp Cell Res       Date:  1982-09       Impact factor: 3.905

6.  A-204197, a new tubulin-binding agent with antimitotic activity in tumor cell lines resistant to known microtubule inhibitors.

Authors:  S K Tahir; E K Han; B Credo; H S Jae; J A Pietenpol; C D Scatena; J R Wu-Wong; D Frost; H Sham; S H Rosenberg; S C Ng
Journal:  Cancer Res       Date:  2001-07-15       Impact factor: 12.701

7.  Cell-based assays for profiling activity and safety properties of cancer drugs.

Authors:  Weiwei Li; Marilyn S Lam; Andrew Birkeland; Angela Riffel; Leticia Montana; Mark E Sullivan; Joseph M Post
Journal:  J Pharmacol Toxicol Methods       Date:  2006-03-27       Impact factor: 1.950

8.  Visualization of assembled and disassembled microtubule protein by double label fluorescence microscopy.

Authors:  D F Albertini; J I Clark
Journal:  Cell Biol Int Rep       Date:  1981-04

9.  PAMPA--a drug absorption in vitro model. 5. Unstirred water layer in iso-pH mapping assays and pKa(flux)--optimized design (pOD-PAMPA).

Authors:  Jeffrey A Ruell; Konstantin L Tsinman; Alex Avdeef
Journal:  Eur J Pharm Sci       Date:  2003-12       Impact factor: 4.384

10.  Fluorescein colchicine. Synthesis, purification, and biological activity.

Authors:  J I Clark; D Garland
Journal:  J Cell Biol       Date:  1978-03       Impact factor: 10.539
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