OBJECTIVE: To determine whether high levels of interleukin (IL)-10 can attenuate the production of tumor necrosis factor (TNF)-alpha-induced proinflammatory cytokines in endometriotic stromal cells. DESIGN: Prospective study. SETTING: Department of Ob/Gyn, Tottori University, Japan. PATIENT(S): Thirty-five patients with ovarian endometrioma and ten patients with uterine myoma. INTERVENTION(S): Endometriotic stromal cells were obtained from chocolate cyst linings of ovaries. Endometrial stromal cells obtained from patient with uterine myoma. MAIN OUTCOME MEASURE(S): Expression of IL-10 gene in endometriotic or endometrial stromal cells was determined by real-time reverse-transcriptase polymerase chain reaction (RT-PCR). We performed immunohistochemical staining to find the presence of IL-10 and IL-10 receptors 1 and 2. We examined the effects of TNF-alpha and IL-10 on the expression of IL-6 or IL-8 by real-time RT-PCR and ELISA. We examined the activation of intracellular signal transduction molecules in endometriotic stromal cells by Western blotting. RESULT(S): Addition of IL-10 suppressed the expressions of IL-6 induced by TNF-alpha and IL-10 induced the phosphorylation of STAT3 in endometriotic stromal cells. TNF-alpha induced the expression of phosphorylated ERK1/2, JNK1/2, and I kappaB. Adding IL-10 suppressed the phosphorylation of these signal molecules. CONCLUSION(S): Interleukin-10 attenuates TNF-alpha-induced IL-6 synthesis via NF-kappaB and MAPK pathways in endometriotic cells.. Interleukin-10 may play a significant role in the pathogenesis of endometriosis.
OBJECTIVE: To determine whether high levels of interleukin (IL)-10 can attenuate the production of tumor necrosis factor (TNF)-alpha-induced proinflammatory cytokines in endometriotic stromal cells. DESIGN: Prospective study. SETTING: Department of Ob/Gyn, Tottori University, Japan. PATIENT(S): Thirty-five patients with ovarian endometrioma and ten patients with uterine myoma. INTERVENTION(S): Endometriotic stromal cells were obtained from chocolate cyst linings of ovaries. Endometrial stromal cells obtained from patient with uterine myoma. MAIN OUTCOME MEASURE(S): Expression of IL-10 gene in endometriotic or endometrial stromal cells was determined by real-time reverse-transcriptase polymerase chain reaction (RT-PCR). We performed immunohistochemical staining to find the presence of IL-10 and IL-10 receptors 1 and 2. We examined the effects of TNF-alpha and IL-10 on the expression of IL-6 or IL-8 by real-time RT-PCR and ELISA. We examined the activation of intracellular signal transduction molecules in endometriotic stromal cells by Western blotting. RESULT(S): Addition of IL-10 suppressed the expressions of IL-6 induced by TNF-alpha and IL-10 induced the phosphorylation of STAT3 in endometriotic stromal cells. TNF-alpha induced the expression of phosphorylated ERK1/2, JNK1/2, and I kappaB. Adding IL-10 suppressed the phosphorylation of these signal molecules. CONCLUSION(S): Interleukin-10 attenuates TNF-alpha-induced IL-6 synthesis via NF-kappaB and MAPK pathways in endometriotic cells.. Interleukin-10 may play a significant role in the pathogenesis of endometriosis.
Authors: R K Baithalu; S K Singh; A Kumaresan; S Kumar; B R Maharana; S Mallick; T K Mohanty; A K Mohanty Journal: Trop Anim Health Prod Date: 2019-06-14 Impact factor: 1.893
Authors: Marco Sbracia; Brett McKinnon; Fabio Scarpellini; Daniela Marconi; Gabriele Rossi; Cedric Simmilion; Michael D Mueller; Eytan R Barnea; Martin Mueller Journal: PLoS One Date: 2017-09-13 Impact factor: 3.240
Authors: Shan Herath; Sonia T Lilly; Natalia R Santos; Robert O Gilbert; Leopold Goetze; Clare E Bryant; John O White; James Cronin; I Martin Sheldon Journal: Reprod Biol Endocrinol Date: 2009-05-29 Impact factor: 5.211