Synchronous fluorescence determination and molecular modeling of 5-Aminosalicylic acid (5-ASA) interacted with human serum albumin.

In this paper, we proposed a new method for the determination of either human serum albumin (HSA) or 5-Aminosalicylic acid (5-ASA) by synchronous fluorescence spectra and examined the interaction between them using the molecular modeling method under simulative physiological conditions. The optimum conditions of synchronous fluorometric determination of HSA were investigated and the method was successfully applied to the determination of 5-ASA added to serum, urine, and saliva samples. The linear range of the determination of HSA and 5-ASA were 1.60 - 414 microg mL(-1) and 0.76 -22.95 microg mL(-1), the detection limits were 0.552 microg mL(-1) and 0.38 microg mL(-1), respectively. In addition, the effect of various common ions on the determination of HSA with 5-ASA was also discussed at room temperature.