Literature DB >> 18681458

Disposable microscope objective lenses for fluorescence correlation spectroscopy using latex microspheres.

Jérôme Wenger1, Davy Gérard, Heykel Aouani, Hervé Rigneault.   

Abstract

We explore the combination of a latex microsphere with a low NA lens to form a high performance optical system, and enable the detection of single molecules by fluorescence correlation spectroscopy (FCS). Viable FCS experiments at concentrations 1-1000 nM with different objectives costing less than $40 are demonstrated. This offers a simple and low-cost alternative to the conventional complex microscope objectives.

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Year:  2008        PMID： 18681458     DOI： 10.1021/ac801016z

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  8 in total

1.  Single-molecule detection: focusing on the objective.

Authors:  Yuval Ebenstein; Laurent A Bentolila
Journal:  Nat Nanotechnol       Date:  2010-02       Impact factor: 39.213

2.  Colloidal lenses allow high-temperature single-molecule imaging and improve fluorophore photostability.

Authors:  Jerrod J Schwartz; Stavros Stavrakis; Stephen R Quake
Journal:  Nat Nanotechnol       Date:  2009-12-20       Impact factor: 39.213

3.  Enhanced emission of fluorophores on shrink-induced wrinkled composite structures.

Authors:  Himanshu Sharma; Michelle A Digman; Natasha Felsinger; Enrico Gratton; Michelle Khine
Journal:  Opt Mater Express       Date:  2014       Impact factor: 3.442

Review 4.  Photonic methods to enhance fluorescence correlation spectroscopy and single molecule fluorescence detection.

Authors:  Jérome Wenger; Hervé Rigneault
Journal:  Int J Mol Sci       Date:  2010-01-13       Impact factor: 6.208

5.  Two-photon fluorescence correlation spectroscopy with high count rates and low background using dielectric microspheres.

Authors:  Heykel Aouani; Peter Schön; Sophie Brasselet; Hervé Rigneault; Jérôme Wenger
Journal:  Biomed Opt Express       Date:  2010-10-05       Impact factor: 3.732

6.  Experimental imaging properties of immersion microscale spherical lenses.

Authors:  Ran Ye; Yong-Hong Ye; Hui Feng Ma; Lingling Cao; Jun Ma; Frank Wyrowski; Rui Shi; Jia-Yu Zhang
Journal:  Sci Rep       Date:  2014-01-20       Impact factor: 4.379

Review 7.  All-dielectric concentration of electromagnetic fields at the nanoscale: the role of photonic nanojets.

Authors:  Jinlong Zhu; Lynford L Goddard
Journal:  Nanoscale Adv       Date:  2019-11-11

8.  Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications.

Authors:  Eben Olson; Richard Torres; Michael J Levene
Journal:  Biomed Opt Express       Date:  2013-06-11       Impact factor: 3.732
  8 in total

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