OBJECTIVE: To investigate the effects of 50% ethyl alcohol (EtOH) extracts from Danzhi Xiaoyao Pill (, DXP) on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. METHODS: ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC). The effects of DXP on nitric oxide (NO) production were tested by lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages using the Griess reaction. RESULTS: The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10, 0.25 and 0.50 mg/mL dose-dependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820 micro moL Trolox equivalent/g, about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL (P<0.05, P<0.01). CONCLUSION: The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells, with the effect possibly related to its antioxidant activity and the inhibition of NO production.
OBJECTIVE: To investigate the effects of 50% ethyl alcohol (EtOH) extracts from Danzhi Xiaoyao Pill (, DXP) on the proliferation of MCF-7 humanbreast cancer cells and potential mechanisms. METHODS:ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC). The effects of DXP on nitric oxide (NO) production were tested by lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages using the Griess reaction. RESULTS: The 50% EtOHDXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10, 0.25 and 0.50 mg/mL dose-dependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820 micro moL Trolox equivalent/g, about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL (P<0.05, P<0.01). CONCLUSION: The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells, with the effect possibly related to its antioxidant activity and the inhibition of NO production.
Authors: Michael J Campbell; Bobbi Hamilton; Mark Shoemaker; Mary Tagliaferri; Isaac Cohen; Debasish Tripathy Journal: Anticancer Res Date: 2002 Nov-Dec Impact factor: 2.480
Authors: T Akaike; S Fujii; A Kato; J Yoshitake; Y Miyamoto; T Sawa; S Okamoto; M Suga; M Asakawa; Y Nagai; H Maeda Journal: FASEB J Date: 2000-07 Impact factor: 5.191
Authors: Joannie Shen; Ronald Andersen; Paul S Albert; Neil Wenger; John Glaspy; Melissa Cole; Paul Shekelle Journal: BMC Complement Altern Med Date: 2002-08-13 Impact factor: 3.659