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Literature DB >> 18678941

Expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.

Paul R Elliott¹, Daniel Evans, Jacqueline A Greenwood, Peter C E Moody.

Abstract

The classical glycolytic pathway contains an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, with NADP-dependent forms reserved for photosynthetic organisms and archaea. Here, the cloning, expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori is reported; crystals of the protein were grown both in the presence and the absence of NADP.

Entities: Chemical Species

Mesh：

Substances：

Year: 2008 PMID： 18678941 PMCID： PMC2494975 DOI： 10.1107/S1744309108020320

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

18 in total

1. Solvent content of protein crystals.

Authors: B W Matthews
Journal: J Mol Biol Date: 1968-04-28 Impact factor: 5.469

2. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.

Authors: B J Marshall; J R Warren
Journal: Lancet Date: 1984-06-16 Impact factor: 79.321

3. Helicobacter pylori gastritis and primary gastric non-Hodgkin's lymphomas.

Authors: S Eidt; M Stolte; R Fischer
Journal: J Clin Pathol Date: 1994-05 Impact factor: 3.411

4. The Entner-Doudoroff pathway has little effect on Helicobacter pylori colonization of mice.

Authors: Amy E Wanken; Tyrrell Conway; Kathryn A Eaton
Journal: Infect Immun Date: 2003-05 Impact factor: 3.441

Review 5. The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer.

Authors: P B Ernst; B D Gold
Journal: Annu Rev Microbiol Date: 2000 Impact factor: 15.500

1. Expression, purification, crystallization and preliminary X-ray analysis of an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.

Authors: Paul R Elliott; Shabaz Mohammad; Helen J Melrose; Peter C E Moody
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2008-07-26

1 in total

Expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.

1. Solvent content of protein crystals.

2. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.

3. Helicobacter pylori gastritis and primary gastric non-Hodgkin's lymphomas.

4. The Entner-Doudoroff pathway has little effect on Helicobacter pylori colonization of mice.

Review 5. The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer.

6. The Entner-Doudoroff pathway in Helicobacter pylori.

7. Glucose utilization and lactate production by Helicobacter pylori.

8. Structure of holo-glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus at 1.8 A resolution.

9. Glucose phosphorylation in Helicobacter pylori.

Review 10. Scaling and assessment of data quality.

1. Expression, purification, crystallization and preliminary X-ray analysis of an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.