Silk fibroin film from non-mulberry tropical tasar silkworms: A novel substrate for in vitro fibroblast culture.

The silk protein fibroin, isolated from the cocoon of the domesticated mulberry silkworm, Bombyx mori, is used extensively in biomaterial design and in cell and tissue culture. We report here for the first time the potential application of fibroin obtained from the cocoon of non-mulberry tropical silkworm, Antheraea mylitta, as a substrate for in vitro cell culture. The mechanical strength of A. mylitta silk fibers indicates a stronger thread composition. The contact angle of A. mylitta fibroin films suggests that it has lower hydrophilicity and lower solubility in organic solvents compared to B. mori fibroin films. Retention of a secondary structure of fibroin in both A. mylitta and B. mori films is confirmed by Fourier transform infrared analysis. The adherence, growth and proliferation patterns of feline fibroblast cells on A. mylitta fibroin films suggest that this kind of film has a greater ability to support cell growth than B. mori fibroin films and is comparable to that of control. This study demonstrates that, as well as being non-toxic to dermal fibroblast cells, non-mulberry fibroin might be a useful alternative substrate to the more common B. mori fibroin for a variety of biomedical applications.