Adipogenic genes on induction and stabilization of commitment to adipose conversion.

Most late events of adipose conversion are known, but those early events that lead to cell commitment, and important aspects of its mechanism remain unknown. We recently described that, in the absence of any other adipogenic factor, 4h incubation with staurosporine promotes commitment of 3T3-F442A cells to adipogenesis. This commitment consists of two stages; a first stage of 4h induction by staurosporine, and, in the absence of this drug, a second stage of stabilization which becomes completed after 40-48h from staurosporine treatment. Here, we demonstrate that pparg2 gene is expressed early after induction stage but before commitment is stabilized, whereas cebpa is highly expressed during the last part of stabilization stage. A decrease of dlk1 expression, whose down-regulation is indispensable for adipogenesis, began to take place between 24 and 48h of St-Dex incubation started, reaching the lowest levels well into the end of stabilization stage.