| Literature DB >> 18675772 |
Eilyn R Lacy1, Margaret Baker, Michael Brigham-Burke.
Abstract
Monoclonal antibodies are a major subclass of biopharmaceuticals. They are structurally different from other biopharmaceuticals in size and quaternary structure. Here we demonstrate a correlation between chemical stability of antibodies and thermal stability. We show that overall thermal protein stability can be predicted based on the measurement of free sulfhydryl (-SH) content on applying mildly denaturing conditions. We propose that this method can be adapted to a high-throughput screening format and used either as an absolute measure of thermal stability or for ranking a panel of possible variants.Mesh：
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Year: 2008 PMID： 18675772 DOI： 10.1016/j.ab.2008.07.016
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365