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Literature DB >> 18675360

SSM-based electrophysiology.

Patrick Schulz¹, Juan J Garcia-Celma, Klaus Fendler.

Abstract

An assay technique for the electrical characterization of electrogenic transport proteins on solid supported membranes is presented. Membrane vesicles, proteoliposomes or membrane fragments containing the transporter are adsorbed to the solid supported membrane and are activated by providing a substrate or a ligand via a rapid solution exchange. This technique opens up new possibilities where conventional electrophysiology fails like transporters or ion channels from bacteria and from intracellular compartments. Its rugged design and potential for automation make it suitable for drug screening.

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Year: 2008 PMID： 18675360 DOI： 10.1016/j.ymeth.2008.07.002

Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608

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Cited

41 in total

1. Electrophysiological characterization of LacY.

Authors: Juan J Garcia-Celma; Irina N Smirnova; H Ronald Kaback; Klaus Fendler
Journal: Proc Natl Acad Sci U S A Date: 2009-04-21 Impact factor: 11.205

2. Replacement of Lys-300 with a glutamine in the NhaA Na⁺/H⁺ antiporter of Escherichia coli yields a functional electrogenic transporter.

Authors: Miyer Patiño-Ruiz; Manish Dwivedi; Octavian Călinescu; Mehmet Karabel; Etana Padan; Klaus Fendler
Journal: J Biol Chem Date: 2018-11-08 Impact factor: 5.157

SSM-based electrophysiology.

1. Electrophysiological characterization of LacY.

2. Replacement of Lys-300 with a glutamine in the NhaA Na⁺/H⁺ antiporter of Escherichia coli yields a functional electrogenic transporter.

3. Measuring ion channels on solid supported membranes.

4. Direct observation of electrogenic NH4(+) transport in ammonium transport (Amt) proteins.

5. Phosphatidylserine flipping by the P4-ATPase ATP8A2 is electrogenic.

6. Functional Characterization of SLC Transporters Using Solid Supported Membranes.

7. From Gene to Function: Cell-Free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs.

8. Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling.

9. Assaying the proton transport and regulation of UCP1 using solid supported membranes.

10. Solid-supported membrane technology for the investigation of the influenza A virus M2 channel activity.