OBJECTIVE: The purpose of this study was to screen a panel of targeted adenoviruses as vectors for endometriosis gene therapy. STUDY DESIGN: Endometriotic cells were obtained from subjects with ovarian endometriomas. Liver tissues were taken from donors during hepatic transplantation surgery. Human endometriotic cells and liver tissues were transfected by targeted adenoviruses expressing luciferase reporter gene. Luciferase activity that was mediated by each virus was expressed as a percentage of adenovirus serotype 5 (Ad5-CMV-luc) activity. The 2-tailed Studentt test was used to compare the adenovirus data. RESULTS: In endometriotic cells, the adenovirus-RGD (Ad-RGD-luc), adenovirus under secretory leukocyte protease inhibitor promoter (Ad-SLPI-luc), and adenovirus under heparanase promoter (Ad-heparanase-luc) showed significantly higher activity, compared with the adenovirus serotype 5. In liver tissues, adenovirus-survivin (Ad-survivin-luc) and Ad-heparanase-luc had significantly lower activity, compared with adenovirus serotype 5. CONCLUSION: Ad-heparanase-luc showed "endometriosis on, liver off" phenotype and is a promising vector for endometriosis gene therapy.
OBJECTIVE: The purpose of this study was to screen a panel of targeted adenoviruses as vectors for endometriosis gene therapy. STUDY DESIGN: Endometriotic cells were obtained from subjects with ovarian endometriomas. Liver tissues were taken from donors during hepatic transplantation surgery. Human endometriotic cells and liver tissues were transfected by targeted adenoviruses expressing luciferase reporter gene. Luciferase activity that was mediated by each virus was expressed as a percentage of adenovirus serotype 5 (Ad5-CMV-luc) activity. The 2-tailed Studentt test was used to compare the adenovirus data. RESULTS: In endometriotic cells, the adenovirus-RGD (Ad-RGD-luc), adenovirus under secretory leukocyte protease inhibitor promoter (Ad-SLPI-luc), and adenovirus under heparanase promoter (Ad-heparanase-luc) showed significantly higher activity, compared with the adenovirus serotype 5. In liver tissues, adenovirus-survivin (Ad-survivin-luc) and Ad-heparanase-luc had significantly lower activity, compared with adenovirus serotype 5. CONCLUSION: Ad-heparanase-luc showed "endometriosis on, liver off" phenotype and is a promising vector for endometriosis gene therapy.
Authors: A Al-Hendy; A M Magliocco; T Al-Tweigeri; G Braileanu; N Crellin; H Li; T Strong; D Curiel; P J Chedrese Journal: Am J Obstet Gynecol Date: 2000-03 Impact factor: 8.661
Authors: Tyler O Kirby; Angel Rivera; Daniel Rein; Minghui Wang; Ilya Ulasov; Martina Breidenbach; Manjula Kataram; Juan L Contreras; Carlos Krumdieck; Masato Yamamoto; Marianne G Rots; Hidde J Haisma; Ronald D Alvarez; Parameshwar J Mahasreshti; David T Curiel Journal: Clin Cancer Res Date: 2004-12-15 Impact factor: 12.531
Authors: Shannon D Barker; Candace J Coolidge; Anna Kanerva; Tanja Hakkarainen; Masato Yamamoto; Bin Liu; Angel A Rivera; Snehal M Bhoola; Mack N Barnes; Ronald D Alvarez; David T Curiel; Akseli Hemminki Journal: J Gene Med Date: 2003-04 Impact factor: 4.565