AIM: TT virus (TTV) is genetically variable and widespread without apparent pathogenicity; however, its epidemiological features in children were not fully understood, partly because blood sampling is often unacceptable for healthy children. We therefore used saliva specimens to investigate epidemiology of TTV infection in early childhood. METHODS: Saliva samples were collected from 83 1-month-old, 110 4-month-old and 49 42-month-old children. Peripheral blood mononuclear cells (PBMC) and saliva samples were obtained in pairs from 19 healthy adults aged 40 +/- 7 years. TTV DNA was detected and quantified by real-time PCR and classified into five genogroups (G1-G5) by a series of PCRs using genogroup-specific primer pairs. RESULTS: TTV DNA was detected in 6, 34 and 90% of children aged 1, 4 and 42 months, respectively, and in 84% of adults. Comparable levels of TTV DNA were detected in pairs of saliva and PBMC. TTV loads in saliva were much higher in children than in adults. G3 was the most common genogroup in all age groups. The second most prevalent was G4 at 1-4 months of age and G1 thereafter. CONCLUSION: The prevalence of TTV infection reached a plateau at or before 42 months; however, somehow different epidemiologic features were observed among genogroups.
AIM: TT virus (TTV) is genetically variable and widespread without apparent pathogenicity; however, its epidemiological features in children were not fully understood, partly because blood sampling is often unacceptable for healthy children. We therefore used saliva specimens to investigate epidemiology of TTV infection in early childhood. METHODS: Saliva samples were collected from 83 1-month-old, 110 4-month-old and 49 42-month-old children. Peripheral blood mononuclear cells (PBMC) and saliva samples were obtained in pairs from 19 healthy adults aged 40 +/- 7 years. TTV DNA was detected and quantified by real-time PCR and classified into five genogroups (G1-G5) by a series of PCRs using genogroup-specific primer pairs. RESULTS:TTV DNA was detected in 6, 34 and 90% of children aged 1, 4 and 42 months, respectively, and in 84% of adults. Comparable levels of TTV DNA were detected in pairs of saliva and PBMC. TTV loads in saliva were much higher in children than in adults. G3 was the most common genogroup in all age groups. The second most prevalent was G4 at 1-4 months of age and G1 thereafter. CONCLUSION: The prevalence of TTV infection reached a plateau at or before 42 months; however, somehow different epidemiologic features were observed among genogroups.
Authors: Vasiliy I Reshetnyak; Igor V Maev; Alexandr I Burmistrov; Igor A Chekmazov; Tatiana I Karlovich Journal: World J Gastroenterol Date: 2020-04-21 Impact factor: 5.742
Authors: Sabrina Rueschenbaum; Sandra Ciesek; Alexander Queck; Marek Widera; Katharina Schwarzkopf; Bernhard Brüne; Christoph Welsch; Heiner Wedemeyer; Stefan Zeuzem; Andreas Weigert; Christian M Lange Journal: Front Immunol Date: 2021-01-26 Impact factor: 7.561
Authors: Alexandre Mendes Batista; Matheus W Caetano; Maria A Stincarelli; Ana C Mamana; Rodrigo Melim Zerbinati; Dmitry J S Sarmento; Marina Gallottini; Rafael A V Caixeta; José Medina-Pestana; Bengt Hasséus; Louise Zanella; Tania R Tozetto-Mendoza; Simone Giannecchini; Paulo H Braz-Silva Journal: J Oral Microbiol Date: 2021-12-12 Impact factor: 5.474
Authors: Joanna Kaczorowska; Aurelija Cicilionytė; Annet Firouzi Wahdaty; Martin Deijs; Maarten F Jebbink; Margreet Bakker; Lia van der Hoek Journal: Front Microbiol Date: 2022-09-16 Impact factor: 6.064