Literature DB >> 18670917

Rapid diagnosis of infectious laryngotracheitis using a monoclonal antibody-based immunoperoxidase procedure.

J S Guy1, H J Barnes, L G Smith.   

Abstract

An indirect immunoperoxidase (IP) procedure using monoclonal antibody was developed for detection of infectious laryngotracheitis (ILT) virus antigen in frozen tissue sections. This IP procedure was compared with an indirect immunofluorescent antibody (FA) procedure, histo-pathology and virus isolation for detection of ILT virus in tracheas of experimentally infected chickens. Compared with virus isolation, sensitivity and specificity of IP were 72 and 93%, respectively; sensitivity and specificity of FA were 53 and 90%, respectively. Histopathological detection of ILT virus infection was highly specific (98%), but sensitivity was poor (42%). These findings indicate potential usefulness of the IP procedure for ILT diagnosis.

Entities:  

Year:  1992        PMID： 18670917     DOI： 10.1080/03079459208418820

Source DB:  PubMed          Journal:  Avian Pathol        ISSN： 0307-9457            Impact factor:   3.378


  7 in total

Review 1.  Infectious laryngotracheitis virus in chickens.

Authors:  Shan-Chia Ou; Joseph J Giambrone
Journal:  World J Virol       Date:  2012-10-12

2.  Retrospective analysis of infectious laryngotracheitis in backyard chicken flocks in California, 2007-2017, and determination of strain origin by partial ICP4 sequencing.

Authors:  Julia Blakey; Simone Stoute; Beate Crossley; Aslı Mete
Journal:  J Vet Diagn Invest       Date:  2019-04-11       Impact factor: 1.279

3.  Characterization of a coronavirus isolated from a diarrheic foal.

Authors:  J S Guy; J J Breslin; B Breuhaus; S Vivrette; L G Smith
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

4.  In situ hybridization for the detection of infectious laryngotracheitis virus in sections of trachea from experimentally infected chickens.

Authors:  O L Nielsen; K J Handberg; P H Jørgensen
Journal:  Acta Vet Scand       Date:  1998       Impact factor: 1.695

5.  Genetically diverse herpesviruses in South American Atlantic coast seabirds.

Authors:  Claudia Niemeyer; Cíntia Maria Favero; H L Shivaprasad; Marcela Uhart; Cesar Meyer Musso; María Virginia Rago; Rodolfo Pinho Silva-Filho; Paula Lima Canabarro; María Isabel Craig; Valeria Olivera; Ariel Pereda; Paulo Eduardo Brandão; José Luiz Catão-Dias
Journal:  PLoS One       Date:  2017-06-02       Impact factor: 3.240

6.  Effect of Pullet Vaccination on Development and Longevity of Immunity.

Authors:  Emily J Aston; Brian J Jordan; Susan M Williams; Maricarmen García; Mark W Jackwood
Journal:  Viruses       Date:  2019-02-02       Impact factor: 5.048

7.  Infectious laryngotracheitis: Etiology, epidemiology, pathobiology, and advances in diagnosis and control - a comprehensive review.

Authors:  Vasudevan Gowthaman; Sachin Kumar; Monika Koul; Urmil Dave; T R Gopala Krishna Murthy; Palanivelu Munuswamy; Ruchi Tiwari; Kumaragurubaran Karthik; Kuldeep Dhama; Izabela Michalak; Sunil K Joshi
Journal:  Vet Q       Date:  2020-12       Impact factor: 3.320
  7 in total

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